| Electrofusion parameters for nuclear transfer predicted using isofusion contours produced with bovine embryonic cells. | |
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MedLine Citation:
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PMID: 8868243 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Electrofusion is a valuable technique for the nuclear transfer procedure. An enucleated oocyte is electrofused with a blastomere to create a nuclear transfer embryo. The present study constructed isofusion contours after the electrofusion of identical coupled cells that characterized all the bovine embryonic cell types used in nuclear transfer. The intersection of isofusion contours for enucleated oocytes and blastomeres provided the parameters for electrofusion during nuclear transfer. Blastomeres isolated from in vitro produced embryos 3-6 days after (in vitro fertilization) were electrofused with oocytes enucleated by centrifugation (85, 87, 89, and 73% electrofusion, respectively). The cleavage (46, 40, 37, and 28%, respectively) of the nuclear transfer embryos produced a trend that decreased as the age of the blastomeres increased. The isofusion contours provided information about the interaction between different cell types in an electric field, and gave precise electrofusion parameters for a range of bovine embryonic cell types used in nuclear transfer. |
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Authors:
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B G Tatham; K J Giliam; A O Trounson |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular reproduction and development Volume: 43 ISSN: 1040-452X ISO Abbreviation: Mol. Reprod. Dev. Publication Date: 1996 Mar |
Date Detail:
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Created Date: 1996-12-05 Completed Date: 1996-12-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8903333 Medline TA: Mol Reprod Dev Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 306-12 Citation Subset: IM |
Affiliation:
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Institute of Reproduction and Development, Monash University, Clayton, Victoria, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastomeres / physiology* Cattle Cell Fusion Cytological Techniques Nuclear Transfer Techniques* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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