Document Detail

Electrofusion of myeloma cells on the single cell level. Fusion under sterile conditions without proteolytic enzyme treatment.
MedLine Citation:
PMID:  6414845     Owner:  NLM     Status:  MEDLINE    
A technique is presented which allows electrofusion of single cells under sterile conditions. The electrofusion chamber is placed in a Petri dish. Before a droplet of the fusion medium is pipetted between the electrodes, the chamber is completely covered with vaseline, which prevents the fusion medium evaporating. Additionally, the fusion chamber is treated with solutions containing poly(L)-lysine and pronase which results in a decreased movement of the cells on the glass between the electrodes and which allows electrofusion without any proteolytic pretreatment.
J Vienken; U Zimmermann; M Fouchard; D Zagury
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  163     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1983 Oct 
Date Detail:
Created Date:  1983-12-17     Completed Date:  1983-12-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  54-6     Citation Subset:  IM    
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MeSH Terms
Cell Fusion*
Cell Line
Culture Techniques / methods
Hybridomas / physiopathology
Plasmacytoma / physiopathology*
Reg. No./Substance:
56-87-1/Lysine; EC 3.4.24.-/Pronase

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