Document Detail

Electrofusion generates diverse DC-tumour cell hybrids for cancer immunotherapy.
MedLine Citation:
PMID:  15161046     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Hybrid cells generated from dendritic cells (DC) and tumour cells provide tumour-associated antigens (TAA) in a polyvalent mode. The present study was designed to investigate the hybrid cell generation by dendritic cells and different tumour cell lines to establish an electrofusion protocol with an optimal fusion setting. MATERIALS AND METHODS: Hybrid cells from mature DC and tumour cells were generated by electrofusion. Fusion efficiency was determined by flow cytometry, as well as by light and fluorescence microscopy analyses. RESULTS: The gradual electrofusion process constituted different human dendritic cell tumour cell hybrids of high diversity depending on electrical and non-electrical parameters. Factors influencing fusion frequency were determined by specific cell staining with mAbs, FACS analysis and trypan blue dye exclusion. CONCLUSION: Increased fusion efficiency was associated with reduced viability. The protocol presented in this work might be helpful for future fusion studies as a prerequisite for comparable in vitro and human vaccination trials.
Jan B Weise; Felix Hilpert; Axel Heiser; Tibor Görögh; Jens E Meyer; Joerg Weimer; Christoph Krüger; Andreas Rosenau; Wilm Wieckhorst; Norbert Arnold; Jacobus Pfisterer; Dietrich Kabelitz; Steffen Maune
Related Documents :
10233966 - Suppression of a fusion defect by second site mutations in the ecotropic murine leukemi...
204066 - Somatic cell hybrids between friend erythroleukemia cells and mouse hepatoma cells.
2447096 - Chinese hamster ovary cell lysosomes rapidly exchange contents.
564366 - Purification of mouse cell interferon induced by isotopically labelled double-stranded ...
215506 - Ultrastructural and enzyme histochemical study of ovarian hilar cells in women and thei...
2543186 - Effects of calcitonin gene-related peptide on cyclic amp formation in chicken, rat, and...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  24     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2004 Mar-Apr
Date Detail:
Created Date:  2004-05-26     Completed Date:  2004-07-19     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  929-34     Citation Subset:  IM    
Department of Otorhinolaryngology, Head and Neck Surgery, University of Kiel, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antigens, Neoplasm / immunology
Carcinoma, Squamous Cell / immunology*,  pathology*
Cell Fusion / methods*
Dendritic Cells / cytology*,  immunology*
Flow Cytometry
HLA-DR Antigens / immunology
Hybrid Cells / cytology,  immunology
Immunotherapy, Adoptive / methods*
Laryngeal Neoplasms / immunology*,  pathology*
Reg. No./Substance:
0/Antigens, Neoplasm; 0/HLA-DR Antigens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Focal adhesion kinase interacts with the transcriptional coactivator FHL2 and both are overexpressed...
Next Document:  Three-dimensional culture and multidrug resistance: effects on immune reactivity of MCF-7 cells by m...