Document Detail


Electrofusion generates diverse DC-tumour cell hybrids for cancer immunotherapy.
MedLine Citation:
PMID:  15161046     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Hybrid cells generated from dendritic cells (DC) and tumour cells provide tumour-associated antigens (TAA) in a polyvalent mode. The present study was designed to investigate the hybrid cell generation by dendritic cells and different tumour cell lines to establish an electrofusion protocol with an optimal fusion setting. MATERIALS AND METHODS: Hybrid cells from mature DC and tumour cells were generated by electrofusion. Fusion efficiency was determined by flow cytometry, as well as by light and fluorescence microscopy analyses. RESULTS: The gradual electrofusion process constituted different human dendritic cell tumour cell hybrids of high diversity depending on electrical and non-electrical parameters. Factors influencing fusion frequency were determined by specific cell staining with mAbs, FACS analysis and trypan blue dye exclusion. CONCLUSION: Increased fusion efficiency was associated with reduced viability. The protocol presented in this work might be helpful for future fusion studies as a prerequisite for comparable in vitro and human vaccination trials.
Authors:
Jan B Weise; Felix Hilpert; Axel Heiser; Tibor Görögh; Jens E Meyer; Joerg Weimer; Christoph Krüger; Andreas Rosenau; Wilm Wieckhorst; Norbert Arnold; Jacobus Pfisterer; Dietrich Kabelitz; Steffen Maune
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  24     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2004 Mar-Apr
Date Detail:
Created Date:  2004-05-26     Completed Date:  2004-07-19     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  929-34     Citation Subset:  IM    
Affiliation:
Department of Otorhinolaryngology, Head and Neck Surgery, University of Kiel, Germany. jweise@hno.uni-kiel.de
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MeSH Terms
Descriptor/Qualifier:
Antigens, Neoplasm / immunology
Carcinoma, Squamous Cell / immunology*,  pathology*
Cell Fusion / methods*
Dendritic Cells / cytology*,  immunology*
Flow Cytometry
HLA-DR Antigens / immunology
Humans
Hybrid Cells / cytology,  immunology
Immunotherapy, Adoptive / methods*
Laryngeal Neoplasms / immunology*,  pathology*
Chemical
Reg. No./Substance:
0/Antigens, Neoplasm; 0/HLA-DR Antigens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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