| Electrochromic responses of bacteriochlorophyll absorbance bands in purified light-harvesting complexes of Rhodobacter capsulatus reconstituted into liposomes. | |
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MedLine Citation:
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PMID: 8369338 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Purified light harvesting complexes I and II (LHI and LHII) from Rhodobacter capsulatus were purified and separately incorporated into liposomes. Electrochromic absorbance changes of bacteriochlorophyll bands in the proteoliposomes in response to K(+)-diffusion potentials were recorded. In LHII proteoliposomes the application of positive-inside potentials led to red shifts in the bacteriochlorophyll absorbance bands centered at 377, 801 and 858 nm. Negative-inside potentials caused blue shifts of these bands. Electrochromism of the 590 nm band was too small to detect. The band at 858 nm was considerably more electrochromic than that at 801 nm. Electrochromic absorbance changes measured at 865-850 nm were linear with the applied diffusion potential. In LHI proteoliposomes positive-inside diffusion potentials caused red shifts of the bands centred at 374 nm and 880 nm. At 880 nm the response was linear with the applied diffusion potential and was equivalent in amplitude to that of the 858 nm band in LHII proteoliposomes. If it is assumed that the permanent dipole moment differences between the ground state and excited state of B800, B850 and B870 are similar and that polarisability effects are negligible, it follows that (a) the plane of the bacteriochlorin ring of B850 is more perpendicular to the membrane plane than that of B800 and (b) the orientations of B850 and B870 relative to the membrane plane are similar. |
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Authors:
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J B Jackson; M G Goodwin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1144 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1993 Sep |
Date Detail:
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Created Date: 1993-10-12 Completed Date: 1993-10-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 199-203 Citation Subset: IM |
Affiliation:
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School of Biochemistry, University of Birmingham, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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chemistry* Bacteriochlorophylls / analysis* Electromagnetic Fields Light-Harvesting Protein Complexes* Liposomes / chemistry Photosystem II Protein Complex* Pigments, Biological / chemistry* Proteolipids / chemistry Rhodobacter capsulatus / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Bacteriochlorophylls; 0/Light-Harvesting Protein Complexes; 0/Liposomes; 0/Photosystem II Protein Complex; 0/Pigments, Biological; 0/Proteolipids; 0/protein complex B800-850, Rhodobacter |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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