| Electrochemistry and chemiluminescence techniques compared in the detection of NADPH oxidase activity in phagocyte cells. | |
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MedLine Citation:
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PMID: 19084665 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several methodologies have been used in clinical chemistry for real-time assessment of NADPH oxidase primary product superoxide anion which dismutases to hydrogen peroxide. Among these methodologies, isoluminol chemiluminescence (CL) is considered to be one of the more sensitive and reliable techniques for the assessment of NADPH oxidase activity in neutrophils. The electrochemical technique was recently designed and also applied for real-time detection of NADPH oxidase activity in neutrophils but its reliability and sensitivity has not been investigated so far. In this study, isoluminol CL and electrochemical techniques were investigated and compared by monitoring the generation of superoxide and hydrogen peroxide in both PLB 985 cell line differentiated into neutrophil-like cells and human neutrophils. The electrochemical technique was shown to be as sensitive as that of CL and able to detect the reactive oxygen species (ROS) release of as low as 500 cells. Thus, the electrochemical technique could be used as an alternative to optical techniques for the evaluation of extracellular ROS in phagocyte cells. |
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Authors:
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A Ashkenazi; K Abu-Rabeah; R S Marks |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-30 |
Journal Detail:
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Title: Talanta Volume: 77 ISSN: 1873-3573 ISO Abbreviation: Talanta Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2008-12-16 Completed Date: 2009-06-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2984816R Medline TA: Talanta Country: England |
Other Details:
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Languages: eng Pagination: 1460-5 Citation Subset: IM |
Affiliation:
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Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva, Israel. ashkenaa@bgu.ac.il |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chemiluminescent Measurements
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methods* Electrochemistry / methods* Humans Hydrogen Peroxide / analysis Kinetics Luminescence Models, Chemical NADPH Oxidase / analysis* Neutrophils / metabolism Phagocytes / metabolism* Reactive Oxygen Species Sensitivity and Specificity Superoxides / metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Reactive Oxygen Species; 11062-77-4/Superoxides; 7722-84-1/Hydrogen Peroxide; EC 1.6.3.1/NADPH Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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