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Electrochemiluminescent disposable cholesterol biosensor based on avidin-biotin assembling with the electroformed luminescent conducting polymer poly(luminol-biotinylated pyrrole).
MedLine Citation:
PMID:  23140959     Owner:  NLM     Status:  In-Data-Review    
An electrochemiluminescent cholesterol disposable biosensor has been prepared by the formation of assembled layers on gold screen-printed cells. The detection layer is based on the electro-formation of new luminol copolymers with different synthesized biotinylated pyrroles prepared by click-chemistry, offering a new transduction layer with new electroluminescent properties on biosensors. The electrochemiluminescence (ECL) luminol copolymers are electroformed by cyclic voltammetry (five cycles) at pH 7.0 uses a10(-3)M biotinylated pyrrole-luminol ratio of 1:10 in PBS buffer. With respect to the recognition layer, cholesterol oxidase was biotinylated by incubation with biotin vinyl sulfone, and immobilized on the copolymer by avidin-biotin interaction. The analytical signal of the biosensor is the ECL enzymatic initial rate working in chronoamperometric mode at 0.5V excitation potential with 10s between pulses at pH 9.5. The disposable device offers a cholesterol linear range from 1.5×10(-5)M to 8.0×10(-4)M with a limit of detection of 1.47×10(-5)M and accuracy of 7.9% for 9.0×10(-5)M and 14.1% for 2.0×10(-4)M, (n=5). Satisfactory results were obtained for cholesterol determination in serum samples compared to a reference procedure.
J Ballesta-Claver; J Ametis-Cabello; J Morales-Sanfrutos; A Megía-Fernández; M C Valencia-Mirón; F Santoyo-González; L F Capitán-Vallvey
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Publication Detail:
Type:  Journal Article     Date:  2012-10-11
Journal Detail:
Title:  Analytica chimica acta     Volume:  754     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  91-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
ECsens, Department of Analytical Chemistry, Campus Fuentenueva, Faculty of Sciences, University of Granada, E-18071 Granada, Spain.
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