Document Detail

Electrochemical nanobiosensor for express diagnosis of acute myocardial infarction in undiluted plasma.
MedLine Citation:
PMID:  20060282     Owner:  NLM     Status:  MEDLINE    
The myocardial infarction biomarker myoglobin was quantified at the biological level in undiluted plasma using developed electrochemical nanosensors with immobilized anti-myoglobin. Method for cardiac myoglobin detection is based on direct electron transfer between Fe(III)-heme and electrode surface modified with gold nanoparticles/didodecyldimethylammonium bromide (DDAB/Au) and antibodies. The procedure of myoglobin detection was optimized (pH, incubation times and characteristics of electrodes) to express determination of the marker in serum or plasma. Plasma of healthy donors and patients with acute myocardial infarction (AMI) was analyzed using electrochemical immunosensors and RAMP immunoassay. Square wave voltammetry cathodic peak of cardiac myoglobin reduction was found to be proportional to myoglobin quantity in plasma as determined by RAMP. The method proposed does not require signal enhancement or amplification; nor does it require labeled secondary antibodies. Immunosensor has a detection limit of 10 ng/ml (0.56 nM) and a broad range of working concentrations (10-1780 ng/ml; 0.56-100 nM). The whole procedure takes 30 min and can be used for express diagnosis of acute myocardial infarction.
Elena Suprun; Tatiana Bulko; Alexander Lisitsa; Oksana Gnedenko; Alexis Ivanov; Victoria Shumyantseva; Alexander Archakov
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-16
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  25     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-17     Completed Date:  2010-05-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  1694-8     Citation Subset:  IM    
Copyright Information:
(c) 2009 Elsevier B.V. All rights reserved.
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya Street 10, Moscow 119121, Russia.
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MeSH Terms
Biological Markers / analysis
Biosensing Techniques / instrumentation*,  methods
Blood Chemical Analysis / instrumentation
Conductometry / instrumentation*,  methods
Equipment Design
Equipment Failure Analysis
Immunoassay / instrumentation*
Myocardial Infarction / blood*,  diagnosis*
Myoglobin / analysis*
Nanotechnology / instrumentation*
Reproducibility of Results
Sensitivity and Specificity
Reg. No./Substance:
0/Biological Markers; 0/Myoglobin

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