Document Detail


Electrochemical microfluidic chips coupled to magnetic bead-based ELISA to control allowable levels of zearalenone in baby foods using simplified calibration.
MedLine Citation:
PMID:  19918609     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel analytical strategy that couples enzyme-linked immunosorbent assay (ELISA) and electrochemical microfluidic chips to determine the mycotoxin zearalenone (ZEA) in baby foods is presented. The analytical cycles for an ultra-fast analysis of the sample and its sequential fast and simplified calibration were performed in about 200 s plus to ELISA protocol. This route avoided the typical four-parameter logistic curve fit which is a highly time-consuming and laborious procedure. An extremely low concentration level of ZEA (less than 1 ppb) was detected with reliability. This level is 20 times lower than the strictest tolerable limit (20 ppb) for baby foods, making the microfluidic approach the newly anticipated analytical security tool for the future. The reliability of the proposal was demonstrated by accuracy evaluations using a certified reference material and by demonstrating its suitability during the control of the regulatory limits of ZEA in baby foods. In addition, the microfluidic approach allowed sensitivity and the incubation enzymatic reaction to be manipulated in situ.
Authors:
Miriam Herv?s; Miguel Angel L?pez; Alberto Escarpa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-06
Journal Detail:
Title:  The Analyst     Volume:  134     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-17     Completed Date:  2010-02-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  2405-11     Citation Subset:  IM    
Affiliation:
Departamento Qu?mica Anal?tica, Universidad de Alcal?, Ctra. Madrid-Barcelona, Km. 33,600, E-28871, Alcal? de Henares (Madrid), Spain.
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MeSH Terms
Descriptor/Qualifier:
Electrochemical Techniques / instrumentation*,  methods
Enzyme-Linked Immunosorbent Assay / instrumentation*
Food Analysis
Food Contamination / analysis
Humans
Infant
Infant Food / analysis*
Magnetics
Microfluidic Analytical Techniques / instrumentation*
Zearalenone / chemistry*
Chemical
Reg. No./Substance:
17924-92-4/Zearalenone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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