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Electrochemical immunosensor for multiplexed detection of food-borne pathogens using nanocrystal bioconjugates and MWCNT screen-printed electrode.
MedLine Citation:
PMID:  22608454     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Bacterial food poisoning is an ever-present threat that can be prevented with proper care and handling of food products. A disposable electrochemical immunosensor for the simultaneous measurements of common food pathogenic bacteria namely Escherichia coli O157:H7 (E. coli), campylobacter and salmonella were developed. The immunosensor was fabricated by immobilizing the mixture of anti-E. coli, anti-campylobacter and anti-salmonella antibodies with a ratio of 1:1:1 on the surface of the multiwall carbon nanotube-polyallylamine modified screen printed electrode (MWCNT-PAH/SPE). Bacteria suspension became attached to the immobilized antibodies when the immunosensor was incubated in liquid samples. The sandwich immunoassay was performed with three antibodies conjugated with specific nanocrystal (α-E. coli-CdS, α-campylobacter-PbS and α-salmonella-CuS) which has releasable metal ions for electrochemical measurements. The square wave anodic stripping voltammetry (SWASV) was employed to measure released metal ions from bound antibody nanocrystal conjugates. The calibration curves for three selected bacteria were found in the range of 1×10(3)-5×10(5)cellsmL(-1) with the limit of detection (LOD) 400cellsmL(-1) for salmonella, 400cellsmL(-1) for campylobacter and 800cellsmL(-1) for E. coli. The precision and sensitivity of this method show the feasibility of multiplexed determination of bacteria in milk samples.
Authors:
Subramanian Viswanathan; Chinnakkaruppanan Rani; Ja-An Annie Ho
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Publication Detail:
Type:  Journal Article     Date:  2012-03-29
Journal Detail:
Title:  Talanta     Volume:  94     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  315-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
REQUIMTE, Instituto Superior de Engenharia do Porto, R.S. Tomé, 4200-072 Porto, Portugal.
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