| Electrochemical immunoassay on expression of integrin β1 on tumor cells and drug-resistant tumor cells. | |
MedLine Citation:
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PMID: 22776180 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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An electrochemical indirect competitive immunoassay protocol as a promising cytosensing strategy was developed to detect integrin β1 expression on human breast cancer MCF-7 cells and adriamycin-resistant human breast cancer MCF-7 (MCF-7/ADR) cells and quantify the cell number. Integrin α5β1 was adsorbed on the gold-nanoparticle modified glassy carbon electrode to bind integrin β1 monoclonal antibody (anti-CD29 mAb). A sandwich structure was then formed using nanocomposites which consisted of horseradish peroxidase (HRP) labeled anti-antibody and gold nanoparticles. HRP bound on the electrode surface could cause an amperometric response of the hydroquinone-H(2)O(2) system. The assembly of the sandwich structure was inhibited by tumor cells to give decreased enzyme-catalytic signals due to the capture of anti-CD29 mAb by integrin β1 on cell membranes. Under optimal conditions the relative current change (S) was proportional to the cell concentration from 1.6×10(3) to 2.0×10(6)cellsmL(-1) with a detection limit of 700cellsmL(-1). Integrin β1 expression in MCF-7/ADR cells was found to be significantly higher than that in MCF-7 cells, indicating the increased adhesion ability of MCF-7/ADR cells. |
Authors:
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Bei Zhou; Xiuli Xiao; Lili Xu; Lian Zhu; Liang Tan; Hao Tang; Youyu Zhang; Qingji Xie; Shouzhuo Yao |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-6-28 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: - ISSN: 1873-4235 ISO Abbreviation: - Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-7-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Elsevier B.V. All rights reserved. |
Affiliation:
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Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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