| Electrochemical genosensor for detection of human mammaglobin in polymerase chain reaction amplification products of breast cancer patients. | |
| | |
MedLine Citation:
|
PMID: 23341004 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Human mammaglobin (MG) has been found to be the most specific molecular marker for the hematogenous spread of breast cancer cells. In our study, an electrochemical impedance spectroscopic DNA biosensor was established for the detection of MG in breast cancer patients. The working conditions for the biosensor, such as immobilization time, rinse process, and hybridization process, were optimized. Under the optimal conditions, the charge transfer resistance of the proposed DNA biosensor shows excellent correlation with the amount of the complementary oligonucleotides in the range from 1.0 × 10(-9) to 2.0 × 10(-8) M. The detection limit is 5.0 × 10(-10) M. The proposed biosensor was used to detect the polymerase chain reaction amplification products of actual clinical breast cancer samples. The results were compared with that obtained by conventional gel electrophoresis. The results indicate that the electrochemical impedance spectroscopic assay is significantly sensitive and time-saving. The simple strategy described here is expected to be used in clinical application for early diagnosis of breast cancer. |
| | |
Authors:
|
Xiongwei Xu; Xiuhua Weng; Ailin Liu; Qihuang Lin; Changlian Wang; Wei Chen; Xinhua Lin |
Related Documents
:
|
23592884 - The clinical significance of breast mri in the management of ductal carcinoma in situ d... 12685184 - Enhancing recovery of endocervical component on gynecologic cytology specimens processe... 23246694 - Inferring coregulation of transcription factors and micrornas in breast cancer. 20931514 - Diagnostic accuracy of fine needle aspiration cytology of palpable breast masses in ben... 21677874 - Insulin-like growth factor-dependent proliferation and survival of triple-negative brea... 17293114 - Sentinel lymph node biopsy in multiple breast cancer using subareolar injection of the ... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2013-1-23 |
Journal Detail:
|
Title: Analytical and bioanalytical chemistry Volume: - ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-1-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Pharmacy, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, China, xxw0409@sina.com. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: In-situ identification of copper-based green pigments on paintings and manuscripts by reflection FTI...
Next Document: Influence of heteroatom pre-selection on the molecular formula assignment of soil organic matter com...