Document Detail


Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G.
MedLine Citation:
PMID:  23145435     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
APOBEC3G catalyzes deamination of cytosines in HIV-1 genome, and restricts the HIV-1 infection. Here, we propose a picomole-scale assay for the detection of DNA deamination catalyzed by APOBEC3G. Our results show the suitability of the developed method for a time course analysis of enzyme-catalyzed DNA modifications.
Authors:
Junya Chiba; Takahide Kouno; Shun Aoki; Hitoshi Sato; JingYing Zhang; Hiroshi Matsuo; Masahiko Inouye
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-11-12
Journal Detail:
Title:  Chemical communications (Cambridge, England)     Volume:  48     ISSN:  1364-548X     ISO Abbreviation:  Chem. Commun. (Camb.)     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-20     Completed Date:  2013-04-22     Revised Date:  2014-01-10    
Medline Journal Info:
Nlm Unique ID:  9610838     Medline TA:  Chem Commun (Camb)     Country:  England    
Other Details:
Languages:  eng     Pagination:  12115-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biocatalysis
Cytidine Deaminase / metabolism*
DNA / analysis*
DNA Probes / chemistry
Deamination
Electrochemical Techniques*
Ferrous Compounds / chemistry
Gold / chemistry
HIV-1 / genetics,  metabolism
Humans
Isoquinolines / chemistry
Grant Support
ID/Acronym/Agency:
AI073167/AI/NIAID NIH HHS; GM091743/GM/NIGMS NIH HHS; R21 AI073167/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/DNA Probes; 0/Ferrous Compounds; 0/Isoquinolines; 7440-57-5/Gold; 9007-49-2/DNA; EC 3.5.4.5/APOBEC3G protein, human; EC 3.5.4.5/Cytidine Deaminase; JGX76Y85M6/isoquinoline; U96PKG90JQ/ferrocene
Comments/Corrections

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