Document Detail


Electrochemical determination of uric acid at ordered mesoporous carbon functionalized with ferrocenecarboxylic acid-modified electrode.
MedLine Citation:
PMID:  18339534     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ordered mesoporous carbon (OMC) functionalized with ferrocenecarboxylic acid (Fc) was used to modify the glassy carbon (GC) electrode. The characterization of OMC-Fc shows that, after anchoring ferrocene on the mesoporous, ordered mesostructure of the material (OMC-Fc) remains intact and Fc is electrochemically accessible. The obtained OMC-Fc-modified electrode was used to investigate the electrochemical behavior of uric acid (UA). UA oxidation is catalyzed by this electrode in aqueous buffer solution (pH 7.3) with a decrease of 200mV in overpotential compared to GC electrode. The detection and determination of UA in the presence of ascorbic acid (AA), the main interferent, were achieved. The voltammetric signals due to UA and AA were well separated with a potential difference of 308mV, a separation that can allow the simultaneous determination of UA and AA. With amperometric method, at a constant potential of 375mV, the catalytic current of UA versus its concentration shows a good linearity in the range 60-390microM (R=0.998) with a detection limit of 1.8microM (S/N=3). These results are not influenced by the presence of AA in the sample solution. With good stability and reproducibility, the present OMC-Fc-modified electrode was applied in the determination of UA content in urine sample and satisfactory results were obtained.
Authors:
Jean Chrysostome Ndamanisha; Liping Guo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-07
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  23     ISSN:  0956-5663     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-04-29     Completed Date:  2008-08-19     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  1680-5     Citation Subset:  IM    
Affiliation:
Faculty of Chemistry, Northeast Normal University, Changchun 130024, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Biosensing Techniques / instrumentation*
Carbon / chemistry*
Electrochemistry / instrumentation*
Equipment Design
Equipment Failure Analysis
Ferrous Compounds / chemistry*
Microelectrodes*
Porosity
Reproducibility of Results
Sensitivity and Specificity
Uric Acid / analysis*
Chemical
Reg. No./Substance:
0/Ferrous Compounds; 1271-42-7/ferrocenecarboxylic acid; 69-93-2/Uric Acid; 7440-44-0/Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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