Document Detail


Electrochemical determination of glutathione in plasma at carbon nanotubes based screen printed electrodes.
MedLine Citation:
PMID:  23782036     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Glutathione (GSH) is a major endogenous antioxidant highly active in human tissues and plays a key role in controlling cellular thiol redox system, maintaining the immune and detoxification system. The determination of GSH levels in tissue is important to estimate endogenous defenses against oxidative stress. In our study, the multi-walled carbon nanotube modified screen-printed electrodes (MWCNT-SPEs) were used to determine the levels of GSH in trichloroacetic acid (TCA)-treated or untreated samples of rat plasma. It was found that the deproteinization of samples with TCA improved the electrochemically detection of GSH particularly in plasma. Meanwhile, the oxidation of GSH was measured by using differential pulse voltammetry (DPV) method in combination with MWCNT-SPE (n=3), and the detection limit of GSH was found as 0.47 µM (S/N=3). The GSH levels in plasma samples were also measured spectrophotometrically in order to compare the effectiveness of electrochemical method and we obtained a high correlation between the two methods (R2=0.976).
Authors:
Ezgi Turunc; Hakan Karadeniz; Guliz Armagan; Arzum Erdem; Ayfer Yalcin
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-6-17
Journal Detail:
Title:  Combinatorial chemistry & high throughput screening     Volume:  -     ISSN:  1875-5402     ISO Abbreviation:  Comb. Chem. High Throughput Screen.     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-6-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810948     Medline TA:  Comb Chem High Throughput Screen     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Ege University, Faculty of Pharmacy, Analytical Chemistry Department, 35100 Bornova, Izmir, TURKEY. arzum.erdem@ege.edu.tr.
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