Document Detail


Electrochemical detection of pathogen infection using cell chip.
MedLine Citation:
PMID:  17171282     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The immobilized cell using self-assembled synthetic oligopeptide was applied to the electrochemical detection of pathogen infection. Thin films based on cysteine-terminated synthetic oligopeptides were fabricated for the immobilization of HeLa cell on gold (Au) substrate. Layer formation and immobilization of the cell were investigated with surface plasmon resonance (SPR) and electrochemical impedance spectroscopy (EIS). Experimental results showed that the thin film of cysteine-terminated synthetic oligopeptide was successfully fabricated and it could be applied for the immobilization of HeLa cells. The adhered living cell was exposed to E. coli O157:H7, which induced the change of SPR angle and electrochemical impedance signal. The proposed cell immobilization method using self-assembly technique can be applied to construct the cell microarray for on-site pathogen monitoring.
Authors:
J W Choi; W Lee; D B Lee; C H Park; J S Kim; Y H Jang; Y Kim
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-14
Journal Detail:
Title:  Environmental monitoring and assessment     Volume:  129     ISSN:  0167-6369     ISO Abbreviation:  Environ Monit Assess     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-07-16     Completed Date:  2007-08-27     Revised Date:  2009-05-11    
Medline Journal Info:
Nlm Unique ID:  8508350     Medline TA:  Environ Monit Assess     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  37-42     Citation Subset:  IM    
Affiliation:
Department of Chemical and Biomolecular Engineering, Sogang University, 1 Sinsu-Dong, Mapo-Gu, Seoul, 121-742, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma*
Cells, Immobilized
Electrochemistry / methods*
Environmental Monitoring
Epithelial Cells / immunology*
Escherichia coli O157
Korea
Oligopeptides
Chemical
Reg. No./Substance:
0/Oligopeptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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