| Electrochemical detection of pathogen infection using cell chip. | |
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MedLine Citation:
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PMID: 17171282 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The immobilized cell using self-assembled synthetic oligopeptide was applied to the electrochemical detection of pathogen infection. Thin films based on cysteine-terminated synthetic oligopeptides were fabricated for the immobilization of HeLa cell on gold (Au) substrate. Layer formation and immobilization of the cell were investigated with surface plasmon resonance (SPR) and electrochemical impedance spectroscopy (EIS). Experimental results showed that the thin film of cysteine-terminated synthetic oligopeptide was successfully fabricated and it could be applied for the immobilization of HeLa cells. The adhered living cell was exposed to E. coli O157:H7, which induced the change of SPR angle and electrochemical impedance signal. The proposed cell immobilization method using self-assembly technique can be applied to construct the cell microarray for on-site pathogen monitoring. |
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Authors:
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J W Choi; W Lee; D B Lee; C H Park; J S Kim; Y H Jang; Y Kim |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-12-14 |
Journal Detail:
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Title: Environmental monitoring and assessment Volume: 129 ISSN: 0167-6369 ISO Abbreviation: Environ Monit Assess Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-07-16 Completed Date: 2007-08-27 Revised Date: 2009-05-11 |
Medline Journal Info:
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Nlm Unique ID: 8508350 Medline TA: Environ Monit Assess Country: Netherlands |
Other Details:
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Languages: eng Pagination: 37-42 Citation Subset: IM |
Affiliation:
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Department of Chemical and Biomolecular Engineering, Sogang University, 1 Sinsu-Dong, Mapo-Gu, Seoul, 121-742, South Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma* Cells, Immobilized Electrochemistry / methods* Environmental Monitoring Epithelial Cells / immunology* Escherichia coli O157 Korea Oligopeptides |
| Chemical | |
Reg. No./Substance:
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0/Oligopeptides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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