| Electrochemical detection in a microfluidic device of oxidative stress generated by macrophage cells. | |
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MedLine Citation:
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PMID: 17268626 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The release of reactive oxygen species (ROS) or reactive nitrogen species (RNS), i.e., the initial phase of oxidative stress, by macrophage cells has been studied by electrochemistry within a microfluidic device. Macrophages were first cultured into a detection chamber containing the three electrodes system and were subsequently stimulated by the microinjection of a calcium ionophore (A23187). Their production of ROS and RNS was then measured by amperometry at the surface of a platinized microelectrode. The fabricated microfluidic device provides an accurate measurement of oxidative release kinetics with an excellent reproducibility. We believe that such a method is simple and versatile for a number of advanced applications based on the detection of biological processes of secretion by a few or even a single living cell. |
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Authors:
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Christian Amatore; Stéphane Arbault; Yong Chen; Cécile Crozatier; Issa Tapsoba |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-10-25 |
Journal Detail:
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Title: Lab on a chip Volume: 7 ISSN: 1473-0197 ISO Abbreviation: Lab Chip Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2007-02-01 Completed Date: 2007-05-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: England |
Other Details:
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Languages: eng Pagination: 233-8 Citation Subset: IM |
Affiliation:
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Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC 8640 "PASTEUR", 24 rue Lhomond, Paris Cedex 05, 75231, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcimycin / pharmacology Calibration Cell Line Electrochemistry / methods* Electrodes Free Radicals Ionophores / pharmacology Macrophages / cytology*, metabolism Mice Microfluidic Analytical Techniques / instrumentation*, methods* Oxidative Stress* Reactive Nitrogen Species Reactive Oxygen Species Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Free Radicals; 0/Ionophores; 0/Reactive Nitrogen Species; 0/Reactive Oxygen Species; 52665-69-7/Calcimycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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