Document Detail


Electrochemical detection of extracellular hydrogen peroxide in Arabidopsis thaliana: A real-time marker of oxidative stress.
MedLine Citation:
PMID:  23057760     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
An electrochemical approach to directly measure the dynamic process of H(2) O(2) release from cultures of Arabidopsis thaliana cells is reported. This approach is based on H(2) O(2 ) oxidation on a Pt electrode in conjunction with continuous measurement of sample pH. For [H(2) O(2) ] < 1 mM, calibration plots were linear and the amperometric response of the electrode was maximum at pH 6. At higher concentrations ([H(2) O(2) ] > 1 mM), the amperometric response can be described by Michaelian-type kinetics and a mathematical expression relating current intensity and pH was obtained to quantitatively determine H(2) O(2) concentration. At pH 5.5, the detection limit of the sensor was 3.1 µM (S/N=3), with a response sensitivity of 0.16 AM(-1) cm(-2) and reproducibility was within 6.1% in the range 1-5 x 10(-3) M (n=5). Cell suspensions under normal physiological conditions had a pH between 5.5-5.7 and H(2) O(2 ) concentrations in the range 7.0-20.5 µM (n=5). The addition of exogenous H(2) O(2) , as well as other potential stress stimuli was made to the cells and the change in H(2) O(2 ) concentrationwas monitored. This real-time quantitative H(2) O(2) analysis is a potential marker for the evaluation of oxidative stress in plant cell cultures. © 2012 Blackwell Publishing Ltd.
Authors:
M I González-Sánchez; L González-Macia; M T Pérez-Prior; E Valero; J Hancock; A J Killard
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-12
Journal Detail:
Title:  Plant, cell & environment     Volume:  -     ISSN:  1365-3040     ISO Abbreviation:  Plant Cell Environ.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9309004     Medline TA:  Plant Cell Environ     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Blackwell Publishing Ltd.
Affiliation:
Departamento de Química-Física, Escuela de Ingenieros Industriales, Universidad de Castilla-La Mancha, Campus Universitario, 02071 Albacete, Spain. Department of Applied Sciences, University of the West England, Coldharbour Lane, Bristol BS16 1QY, United Kingdom.
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