Document Detail


Electrochemical detection of 17beta-estradiol using DNA aptamer immobilized gold electrode chip.
MedLine Citation:
PMID:  17118645     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An electrochemical detection method for chemical sensing has been developed using a DNA aptamer immobilized gold electrode chip. DNA aptamers specifically binding to 17beta-estradiol were selected by the SELEX (Systematic Evolution of Ligands by EXponential enrichment) process from a random ssDNA library, composed of approximately 7.2 x 10(14) DNA molecules. Gold electrode chips were employed to evaluate the electrochemical signals generated from interactions between the aptamers and the target molecules. The DNA aptamer immobilization on the gold electrode was based on the avidin-biotin interaction. The cyclic voltametry (CV) and square wave voltametry (SWV) values were measured to evaluate the chemical binding to aptamer. When 17beta-estradiol interacted with the DNA aptamer, the current decreased due to the interference of bound 17beta-estradiol with the electron flow produced by a redox reaction between ferrocyanide and ferricyanide. In the negative control experiments, the current decreased only mildly due to the presence of other chemicals.
Authors:
Yeon Seok Kim; Ho Sup Jung; Toshihiko Matsuura; Hea Yeon Lee; Tomoji Kawai; Man Bock Gu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-21
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  22     ISSN:  0956-5663     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-02     Completed Date:  2007-06-27     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  2525-31     Citation Subset:  IM    
Affiliation:
College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-701, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Aptamers, Nucleotide / analysis,  chemistry*
Biosensing Techniques / instrumentation*,  methods
Electrochemistry / instrumentation*,  methods
Electrodes*
Equipment Design
Equipment Failure Analysis
Estradiol / analysis*,  chemistry
Gold / chemistry*
Microelectrodes
Oligonucleotide Array Sequence Analysis / instrumentation*,  methods
Reproducibility of Results
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Aptamers, Nucleotide; 50-28-2/Estradiol; 7440-57-5/Gold

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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