| Electrochemical gene sensor arrays prepared using non-contact nanoliter array spotting of gene probes. | |
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MedLine Citation:
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PMID: 20215688 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A capillary-based pitch-variable array spotter, designed to dispense solutions in nanoliter volumes with high precision and density, was used to immobilize gene probes on a microelectrode array chip. Small volumes of the probe solutions were dispensed onto the microelectrodes, without any physical contact between the capillary ends and the electrode surfaces, to fabricate an electrochemical gene sensor array chip. The fabricated gene sensor array chip showed sequence-selective responses, expressed on a pseudocolor scale, to a target DNA sample. It was demonstrated that this dispensing technique provides integrated sensor array chips by dispensing small volumes of solutions of synthesized functional gene probes onto microelectrode array chips, a process not possible with conventional dispensing techniques. |
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Authors:
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Hiroshi Aoki; Akiko Kitajima; Hiroaki Tao |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry Volume: 26 ISSN: 1348-2246 ISO Abbreviation: Anal Sci Publication Date: 2010 |
Date Detail:
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Created Date: 2010-03-10 Completed Date: 2010-06-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8511078 Medline TA: Anal Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 367-70 Citation Subset: IM |
Affiliation:
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National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan. aoki-h@aist.go.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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DNA
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analysis* DNA Probes* Electrochemistry Microelectrodes Oligonucleotide Array Sequence Analysis / methods* |
| Chemical | |
Reg. No./Substance:
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0/DNA Probes; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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