Document Detail


Electroblotting from polyacrylamide gels.
MedLine Citation:
PMID:  18429098     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This unit contains procedures for electrophoretically transferring proteins onto a variety of membranes including polyvinylidene difluoride (PVDF) and nitrocellulose, and derivatized membranes. The choice of membrane type for electrotransfer is dependent on the ultimate application for the blot membrane. An alternate protocol is provided for electroblotting in semidry systems. This unit also describes procedures for eluting proteins from membranes using detergents or acidic extraction with organic solvents.
Authors:
J A Ursitti; J Mozdzanowski; D W Speicher
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in protein science / editorial board, John E. Coligan ... [et al.]     Volume:  Chapter 10     ISSN:  1934-3663     ISO Abbreviation:  -     Publication Date:  2001 May 
Date Detail:
Created Date:  2008-04-22     Completed Date:  2008-05-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101287868     Medline TA:  Curr Protoc Protein Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 10.7     Citation Subset:  IM    
Affiliation:
The Wistar Institute, Philadelphia, Pennsylvania, USA.
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MeSH Terms
Descriptor/Qualifier:
Acrylic Resins*
Blotting, Western / methods*
Collodion
Detergents
Electrophoresis / methods*
Membranes, Artificial
Organic Chemicals
Polyvinyls
Proteins / chemistry
Sequence Analysis, Protein
Solvents
Chemical
Reg. No./Substance:
0/Acrylic Resins; 0/Detergents; 0/Membranes, Artificial; 0/Organic Chemicals; 0/Polyvinyls; 0/Proteins; 0/Solvents; 0/polyacrylamide gels; 24937-79-9/polyvinylidene fluoride; 9004-70-0/Collodion

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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