| Electroactive Multilayer Assemblies of Bilirubin Oxidase and Human Cytochrome C Mutants: Insight in Formation and Kinetic Behavior. | |
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MedLine Citation:
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PMID: 21401056 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Here, we report on cytochrome c/bilirubin oxidase multilayer electrodes with different cytochrome c (cyt c) forms including mutant forms of human cyt c, which exhibit different reaction rates with bilirubin oxidase (BOD) in solution. The multilayer formation via the layer-by-layer technique and the kinetic behavior of the mono (only cyt c) and biprotein (cyt c and BOD) multilayer systems are studied by SPR and cyclic voltammetry. For the layer construction, sulfonated polyaniline is used. The only cyt c containing multilayer electrodes show that the quantity of deposited protein and the kinetic behavior depend on the cyt c form incorporated. In the case of the biprotein multilayer with BOD, it is demonstrated that the catalytic signal chain from the electrode via cyt c to BOD and oxygen can be established with all chosen cyt c forms. However, the magnitude of the catalytic current as well as the kinetic behavior differ significantly. We conclude that the different cytochrome c forms affect three parameters, identified here, to be important for the functionality of the multilayer system: the amount of molecules per layer, which can be immobilized on the electrodes, the cyt c self-exchange rate, and the rate constant for the reaction with BOD. |
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Authors:
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Franziska Wegerich; Paola Turano; Marco Allegrozzi; Helmuth Möhwald; Fred Lisdat |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-14 |
Journal Detail:
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Title: Langmuir : the ACS journal of surfaces and colloids Volume: - ISSN: 1520-5827 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882736 Medline TA: Langmuir Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Max Planck Institute of Colloids and Interfaces , Am Mühlenberg 1, 14424 Potsdam-Golm, Germany; |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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