Document Detail


Electro-sensitive Polyacrylic acid/Fibrin Hydrogel facilities Cell seeding and Alignment.
MedLine Citation:
PMID:  22515272     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
3D Cell culture and conditioning is an effective means to guide cell distribution and patterning for tissue engineered constructs such as vascular grafts. Poly acrylic acid is known as an electro-responsive polymer, capable to transform environmental stimuli like electrical energy, to mechanical forces. In this study, we developed an electro-sensitive and biocompatible hydrogel based smart device composed of Acrylic acid and Fibrin as a tissue engineered construct to mechanically stimulate cells. Structural properties of the hydrogel were assessed by FTIR-ATR, scanning electron microscopy, prosimetry and swelling measurement. Distribution and alignment of porcine smooth muscle cells (pSMCs) seeded on the surface of lyophilized hydrogels were evaluated and quantified by two-photon laser scanning microscopy. Smooth muscle cell tissue constructs exposed to 2 hrs of pulsatile electrical stimulation showed significantly enhanced cell penetration and alignment due to dynamic changes produced by alternative swelling and deswelling, in comparison with static samples. Based on the results, this hydrogel under electrical stimulation works as a mechanical pump, which can direct SMC alignment and facilitate infiltration and distribution of cells throughout the structure.
Authors:
Nastaran Rahimi; Daniel G Molin; Thomas Jan Cleij; Marc A M J van Zandvoort; Mark J Post
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-19
Journal Detail:
Title:  Biomacromolecules     Volume:  -     ISSN:  1526-4602     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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