Document Detail

Electro-physiological measurements in cultured cellular spheroids.
MedLine Citation:
PMID:  6846019     Owner:  NLM     Status:  MEDLINE    
Human Glioma U-118 MG and hamster V-79-379A spheroids were used as models of the nodular structure often seen in poorly vascularized regions of solid tumours. K+, Ca2+ activities and membrane potentials were measured in the spheroids, using microelectrodes. Both stable and peak values were obtained. All values were included in the analysis to allow an accurate estimate of the relative number of electro-physiologically active cells at different depths in the spheroids. The relative number of active cells decreased drastically with depth although most cells in the corresponding regions looked morphologically viable. The amplitude values of potentials and K+ activities showed, however, no significant variations with depth. The Ca2+ activity varied largely between individual cells. The relative number of proliferative cells decreased with depth in the spheroids, in parallel to the decrease in the number of electro-physiologically active cells. The results indicate that the fraction of cells, in poorly vascularized regions, having normal metabolism might be fewer than what can be estimated only on a morphological basis. Thus, the amount of cells with the capacity to contribute to growth might be overestimated when inspecting histological sections.
H Acker; J Carlsson; C G Stålnacke
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta pathologica, microbiologica, et immunologica Scandinavica. Section A, Pathology     Volume:  91     ISSN:  0108-0164     ISO Abbreviation:  Acta Pathol Microbiol Immunol Scand A     Publication Date:  1983 Mar 
Date Detail:
Created Date:  1983-06-23     Completed Date:  1983-06-23     Revised Date:  2009-06-04    
Medline Journal Info:
Nlm Unique ID:  8206622     Medline TA:  Acta Pathol Microbiol Immunol Scand A     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  151-60     Citation Subset:  IM    
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MeSH Terms
Calcium / physiology
Cell Line
Glioma / physiopathology*
Lung / cytology
Membrane Potentials
Neoplasms, Experimental / physiopathology
Potassium / physiology
Reg. No./Substance:
7440-09-7/Potassium; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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