| Electrically regulated cellular morphological and cytoskeletal changes on an optically transparent electrode. | |
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MedLine Citation:
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PMID: 2744199 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Electrically regulated morphological and cytoskeletal changes of HeLa cells were studied on an optically transparent electrode (OTE), on which potential-applied surface cellular behavior and morphogenesis were easily observed. Upon application of a potential, HeLa cells in an OTE exhibited remarkable morphological changes above +0.5 V (vs. Ag/AgCl) and below 0 V. At each potential in this potential range, change in F-actin distribution was observed using a fluorescent probe (rhodamine phalloidin). These results suggest that an electrical field induces a subcellular cytoskeletal change. Electrostimulation of cells with OTE can be a valuable strategy for the manipulation of cultured human cells. |
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Authors:
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M Yaoita; M Aizawa; Y Ikariyama |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Experimental cell biology Volume: 57 ISSN: 0304-3568 ISO Abbreviation: Exp. Cell Biol. Publication Date: 1989 |
Date Detail:
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Created Date: 1989-08-15 Completed Date: 1989-08-15 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7701827 Medline TA: Exp Cell Biol Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 43-51 Citation Subset: IM |
Affiliation:
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Department of Bioengineering, Tokyo Institute of Technology, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism* Cell Membrane / ultrastructure* Cytoskeleton / ultrastructure* Electric Stimulation Hela Cells Humans Kinetics Microelectrodes |
| Chemical | |
Reg. No./Substance:
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0/Actins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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