Document Detail


Electrically regulated cellular morphological and cytoskeletal changes on an optically transparent electrode.
MedLine Citation:
PMID:  2744199     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Electrically regulated morphological and cytoskeletal changes of HeLa cells were studied on an optically transparent electrode (OTE), on which potential-applied surface cellular behavior and morphogenesis were easily observed. Upon application of a potential, HeLa cells in an OTE exhibited remarkable morphological changes above +0.5 V (vs. Ag/AgCl) and below 0 V. At each potential in this potential range, change in F-actin distribution was observed using a fluorescent probe (rhodamine phalloidin). These results suggest that an electrical field induces a subcellular cytoskeletal change. Electrostimulation of cells with OTE can be a valuable strategy for the manipulation of cultured human cells.
Authors:
M Yaoita; M Aizawa; Y Ikariyama
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental cell biology     Volume:  57     ISSN:  0304-3568     ISO Abbreviation:  Exp. Cell Biol.     Publication Date:  1989  
Date Detail:
Created Date:  1989-08-15     Completed Date:  1989-08-15     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7701827     Medline TA:  Exp Cell Biol     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  43-51     Citation Subset:  IM    
Affiliation:
Department of Bioengineering, Tokyo Institute of Technology, Japan.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism*
Cell Membrane / ultrastructure*
Cytoskeleton / ultrastructure*
Electric Stimulation
Hela Cells
Humans
Kinetics
Microelectrodes
Chemical
Reg. No./Substance:
0/Actins

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