| Electrically detected displacement assay (EDDA): a practical approach to nucleic acid testing in clinical or medical diagnosis. | |
| | |
MedLine Citation:
|
PMID: 18425643 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This paper introduces the electrically detected displacement assay (EDDA), a electrical biosensor detection principle for applications in medical and clinical diagnosis, and compares the method to currently available microarray technologies in this field. The sensor can be integrated into automated systems of routine diagnosis, but may also be used as a sensor that is directly applied to the polymerase chain reaction (PCR) reaction vessel to detect unlabeled target amplicons within a few minutes. Major aspects of sensor assembly like immobilization procedure, accessibility of the capture probes, and prevention from nonspecific target adsorption, that are a prerequisite for a robust and reliable performance of the sensor, are demonstrated. Additionally, exemplary results from a human papillomavirus assay are presented. |
| | |
Authors:
|
P Liepold; T Kratzmüller; N Persike; M Bandilla; M Hinz; H Wieder; H Hillebrandt; E Ferrer; G Hartwich |
Related Documents
:
|
17660633 - Ending in wonder: replacing technology with revelation in margaret edson's w;t. 7927153 - Innovation in medical technology: reading the indicators. 14573713 - Healthy worker effect and changes in respiratory symptoms and lung function in hairdres... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-04-20 |
Journal Detail:
|
Title: Analytical and bioanalytical chemistry Volume: 391 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2008 Jul |
Date Detail:
|
Created Date: 2008-06-20 Completed Date: 2008-07-31 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
|
Languages: eng Pagination: 1759-72 Citation Subset: IM |
Affiliation:
|
FRIZ Biochem Gesellschaft für Bioanalytik mbH, 82061, Neuried, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Base Sequence Biological Assay / methods* Biosensing Techniques / methods* Electricity Genotype Humans Microarray Analysis / methods Molecular Diagnostic Techniques / methods* Molecular Sequence Data Nucleic Acids / analysis*, genetics Oligonucleotide Probes / genetics Papillomaviridae / genetics, isolation & purification* Papillomavirus Infections / diagnosis* Polymerase Chain Reaction / methods Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
|
0/Nucleic Acids; 0/Oligonucleotide Probes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Certification of standard reference materials containing bitter orange.
Next Document: Determination of fluoroquinolone antibiotics in hospital and municipal wastewaters in Coimbra by liq...