| Electrical properties of mammalian lens epithelial gap junction channels. | |
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MedLine Citation:
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PMID: 8056517 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To establish an "electrical fingerprint" for the gap junction channels between mammalian lens epithelial cells. METHODS: The double whole cell patch clamp technique was applied to isolated cell pairs obtained from mouse lens epithelium and a continuous cell line of lens epithelial cells derived from the sheep lens (SLE 2.1). RESULTS: The junctional conductance in mouse lens epithelial cells and in cultured SLE 2.1 cells was found to be moderately voltage dependent. SLE 2.1 cells were analyzed in more detail. The voltage dependence could be described by a Boltzmann distribution with Vo = +/- 63.1 mV and Gmin = 0.34. In cell pairs that exhibited spontaneously low junctional conductance, single channel events could be distinguished. Single gap junction channel currents had a linear current-voltage relationship. A frequency histogram of single channel conductances from eight cell pairs had three major peaks of 35, 60 and 97 pS. CONCLUSION: The electrical properties of gap junction channels between mammalian lens epithelial cells were virtually identical to those previously reported for transfected cell lines expressing connexin43. The authors' physiological data are therefore in agreement with molecular studies that have identified connexin43 as the major connexin of lens epithelial cells. |
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Authors:
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P J Donaldson; M Roos; C Evans; E Beyer; J Kistler |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 35 ISSN: 0146-0404 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 1994 Aug |
Date Detail:
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Created Date: 1994-09-12 Completed Date: 1994-09-12 Revised Date: 2010-08-25 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3422-8 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Auckland, New Zealand. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Communication Cells, Cultured Connexin 43 / physiology Electrophysiology Epithelium / physiology Fluorescent Antibody Technique Gap Junctions / physiology* Lens, Crystalline / physiology* Membrane Potentials / physiology Mice Sheep |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Connexin 43 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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