Document Detail

Electrical excitability of cultured adrenal chromaffin cells.
MedLine Citation:
PMID:  1034699     Owner:  NLM     Status:  MEDLINE    
1. Adult human and gerbil adrenal medullary cells were maintained in dissociated cell culture and studied by micro-electrode penetration. 2. In the best recordings, chromaffin cell transmembrane potentials exceeded -50mV. 3. Chromaffin cells were capable of generating all-or-nothing over-shooting action potentials, similar to those generated by sympathetic neurones. 4. The action potentials were blocked by tetrodotoxin (TTX, 10(-6)g/ml.) but were not blocked by removal of Ca or by CoCl2 (10 mM). We conclude that the action potentials are probably generated by a Na mechanism. 5. Chromaffin cells are depolarized by the iontophoretic application of acetylcholine (ACh). This depolarization was accompanied by an increased membrane conductance and could trigger action potentials. 6. Action potentials were also found in cells in fresh slices of gerbil adrenal medullae.
B Biales; M Dichter; A Tischler
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of physiology     Volume:  262     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  1976 Nov 
Date Detail:
Created Date:  1977-03-15     Completed Date:  1977-03-15     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  743-53     Citation Subset:  IM    
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MeSH Terms
Acetylcholine / pharmacology
Action Potentials* / drug effects
Adrenal Medulla / cytology,  physiology*
Cells, Cultured
Membrane Potentials* / drug effects
Sodium / metabolism
Tetrodotoxin / pharmacology
Reg. No./Substance:
4368-28-9/Tetrodotoxin; 51-84-3/Acetylcholine; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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