Document Detail


Elastomeric negative acoustic contrast particles for affinity capture assays.
MedLine Citation:
PMID:  23331264     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This report describes the development of elastomeric capture microparticles (ECμPs) and their use with acoustophoretic separation to perform microparticle assays via flow cytometry.We have developed simple methods to form ECμPs by cross-linking droplets of common commercially available silicone precursors in suspension followed by surface functionalization with biomolecular recognition reagents. The ECμPs are compressible particles that exhibit negative acoustic contrast in ultrasound when suspended in aqueous media, blood serum, or diluted blood. In this study, these particles have been functionalized with antibodies to bind prostate specific antigen and immunoglobulin (IgG). Specific separation of the ECμPs from blood cells is achieved by flowing them through a microfluidic acoustophoretic device that uses an ultrasonic standing wave to align the blood cells, which exhibit positive acoustic contrast, at a node in the acoustic pressure distribution while aligning the negative acoustic contrast ECμPs at the antinodes. Laminar flow of the separated particles to downstream collection ports allows for collection of the separated negative contrast (ECμPs) and positive contrast particles (cells). Separated ECμPs were analyzed via flow cytometry to demonstrate nanomolar detection for prostate specific antigen in aqueous buffer and picomolar detection for IgG in plasma and diluted blood samples. This approach has potential applications in the development of rapid assays that detect the presence of low concentrations of biomarkers in a number of biological sample types.
Authors:
Kevin W Cushing; Menake E Piyasena; Nick J Carroll; Gian C Maestas; Beth Ann López; Bruce S Edwards; Steven W Graves; Gabriel P López
Related Documents :
14998044 - Multilevel cycle of anthropogenic copper.
10375744 - Negative correlation between cyclophilin mrna level in leukocytes of renal allograft re...
24028574 - Biofilm development and the dynamics of preferential flow paths in porous media.
12969514 - Endocannabinoid signalling in the blood of patients with schizophrenia.
8989854 - A new method for measuring nitrogen and gases in blood.
12190404 - Avalanche dynamics in wet granular materials.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-02-05
Journal Detail:
Title:  Analytical chemistry     Volume:  85     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-19     Completed Date:  2013-11-18     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2208-15     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology
Biological Markers / analysis,  blood
Dimethylpolysiloxanes / chemistry
Flow Cytometry / methods*
Humans
Immunoglobulin G / blood
Mice
Microfluidic Analytical Techniques
Microspheres*
Polymers / chemical synthesis,  chemistry*
Prostate-Specific Antigen / analysis*
Swine
Grant Support
ID/Acronym/Agency:
P41 RR001315/RR/NCRR NIH HHS; R25 GM060201/GM/NIGMS NIH HHS; R33 RR020064/RR/NCRR NIH HHS; RR001315/RR/NCRR NIH HHS; RR020064/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Biological Markers; 0/Dimethylpolysiloxanes; 0/Immunoglobulin G; 0/Polymers; 0/elastomeric; EC 3.4.21.77/Prostate-Specific Antigen
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  On the Origin of Thermal Untwisting of DNA.
Next Document:  Assessing the Impact of Pilot School Snack Programs on Milk and Alternatives Intake in 2 Remote Fir...