Document Detail


Elastin metabolism is disrupted in patients after allogeneic hematopoietic stem cell transplantation (alloHSCT) for acute and chronic myeloid leukemia.
MedLine Citation:
PMID:  18971876     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The potential role of elastin in patients with myeloid leukemia treated with hematopoietic stem cell transplantation (HSCT) has not been investigated so far. The objective of the study was to evaluate elastin metabolism before and at two time-points after HSCT. MATERIAL/METHODS: Forty patients (22 male and 18 female, median age: 34 years, range: 14-54) were included. The diagnoses were acute myeloid leukemia (AML, n=25) and chronic myeloid leukemia (CML, n=15). Busulfan and cyclophosphamide (4+2) were administered as conditioning. Graft-versus-host disease (GVHD) prophylaxis consisted of cyclosporine and methotrexate. Twenty-three patients experienced acute GVHD and 17 developed chronic GVHD. Plasma elastase activity (EA) and plasma elastin-derived peptide concentration (EDPc) were measured. RESULTS: There were statistically significant differences in EA before HSCT (1.3 U/ml) compared with day +30 (2.9 U/ml) and day +100 (3.2 U/ml) after HSCT (p<0.001 for both). EA was also higher in patients with chronic GVHD than in those who did not develop chronic GVHD. EDPc was significantly higher on days +30 (49.3 U/ml) and +100 (57.7 U/ml) after HSCT than on day -10 before HSCT (15.5 U/ml, p<0.001 for both). EDPc was significantly higher in patients with chronic GVHD. CONCLUSIONS: EA and EDPc were increased in patients after HSCT. If the role of elastase in the pathogenesis of GVHD is confirmed, it will be possible to apply inhibitors of elastases in the treatment of this condition in the near future.
Authors:
Grzegorz Helbig; Sławomira Krzemień; Tomasz Francuz; Jerzy Wojnar; Jerzy Hołowiecki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medical science monitor : international medical journal of experimental and clinical research     Volume:  14     ISSN:  1643-3750     ISO Abbreviation:  Med. Sci. Monit.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-30     Completed Date:  2008-12-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9609063     Medline TA:  Med Sci Monit     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  CR584-8     Citation Subset:  IM    
Affiliation:
Department of Hematology and Bone Marrow Transplantation, Silesian Medical University, Katowice, Poland. ghelbig@tlen.pl
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MeSH Terms
Descriptor/Qualifier:
Acute Disease
Adolescent
Adult
Chronic Disease
Elastin / metabolism*
Female
Hematopoietic Stem Cell Transplantation*
Humans
Leukemia, Myeloid / metabolism*,  surgery
Male
Middle Aged
Pancreatic Elastase / metabolism
Peptide Fragments / biosynthesis
Transplantation, Homologous
Young Adult
Chemical
Reg. No./Substance:
0/Peptide Fragments; 9007-58-3/Elastin; EC 3.4.21.36/Pancreatic Elastase

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