| Elastin--lipid interaction action in the arterial wall. Part 1. Extraction of elastin from human aortic intima. | |
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MedLine Citation:
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PMID: 465128 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Elastin was extracted from human aortic plaque and adjacent grossly normal intima by the following methods: (1) 0.1 N NaOH at 100 degrees C, (2) hot NaOH and 0.2 M EDTA, (3) 5 M guanidine--HCl and collagenase, (4) guanidine--collagenase and dithioerythritol--urea--sodium dodecyl sulfate, (5) guanidine--collagenase and EDTA, (6) 10% NaCl and collagenase, and (7) NaCl--collagenase and EDTA. All elastin samples contained small amounts of carbohydrate and hydroxyproline. The lipid content of non-plaque intimal elastin samples was small (2--3%), whereas it increased to 4--6% in plaque intima. The lipid composition of elastin preparations varied significantly with the extraction procedure. Elastin from plaque intima contained significantly more cholesterol (50--60%) and less triglyceride and phospholipid than elastin of non-plaque intima (30--50% cholesterol). The contents of free and esterified cholesterol were comparable in all preparations. The main phospholipid in all samples was sphingomyelin, which comprised between 50 and 80% of the total phospholipid. Compared with NaOH-purified elastin, the other elastin samples were characterized by an increased phosphatidyl--choline content, while they all contained an almost equal amount of phosphatidylethanolamine. In elastin samples from plaque intima, the polar amino acids were increased, whereas cross-linking amino acids were decreased. The polarity and hydroxyproline content of elastin samples were slightly decreased after treatment with EDTA or dithioerythritol--urea--sodium dodecyl sulfate. |
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Authors:
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A Noma; T Takahashi; K Yamada; T Wada |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Atherosclerosis Volume: 33 ISSN: 0021-9150 ISO Abbreviation: Atherosclerosis Publication Date: 1979 May |
Date Detail:
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Created Date: 1979-09-17 Completed Date: 1979-09-17 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0242543 Medline TA: Atherosclerosis Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 29-39 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism Aorta / metabolism* Arteriosclerosis / metabolism* Carbohydrate Metabolism Cholesterol / metabolism Cholesterol Esters / metabolism Elastin / metabolism* Humans Lipid Metabolism* Phospholipids / metabolism Triglycerides / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Cholesterol Esters; 0/Phospholipids; 0/Triglycerides; 57-88-5/Cholesterol; 9007-58-3/Elastin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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