Document Detail

Elastin--lipid interaction action in the arterial wall. Part 1. Extraction of elastin from human aortic intima.
MedLine Citation:
PMID:  465128     Owner:  NLM     Status:  MEDLINE    
Elastin was extracted from human aortic plaque and adjacent grossly normal intima by the following methods: (1) 0.1 N NaOH at 100 degrees C, (2) hot NaOH and 0.2 M EDTA, (3) 5 M guanidine--HCl and collagenase, (4) guanidine--collagenase and dithioerythritol--urea--sodium dodecyl sulfate, (5) guanidine--collagenase and EDTA, (6) 10% NaCl and collagenase, and (7) NaCl--collagenase and EDTA. All elastin samples contained small amounts of carbohydrate and hydroxyproline. The lipid content of non-plaque intimal elastin samples was small (2--3%), whereas it increased to 4--6% in plaque intima. The lipid composition of elastin preparations varied significantly with the extraction procedure. Elastin from plaque intima contained significantly more cholesterol (50--60%) and less triglyceride and phospholipid than elastin of non-plaque intima (30--50% cholesterol). The contents of free and esterified cholesterol were comparable in all preparations. The main phospholipid in all samples was sphingomyelin, which comprised between 50 and 80% of the total phospholipid. Compared with NaOH-purified elastin, the other elastin samples were characterized by an increased phosphatidyl--choline content, while they all contained an almost equal amount of phosphatidylethanolamine. In elastin samples from plaque intima, the polar amino acids were increased, whereas cross-linking amino acids were decreased. The polarity and hydroxyproline content of elastin samples were slightly decreased after treatment with EDTA or dithioerythritol--urea--sodium dodecyl sulfate.
A Noma; T Takahashi; K Yamada; T Wada
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Atherosclerosis     Volume:  33     ISSN:  0021-9150     ISO Abbreviation:  Atherosclerosis     Publication Date:  1979 May 
Date Detail:
Created Date:  1979-09-17     Completed Date:  1979-09-17     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0242543     Medline TA:  Atherosclerosis     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  29-39     Citation Subset:  IM    
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MeSH Terms
Amino Acids / metabolism
Aorta / metabolism*
Arteriosclerosis / metabolism*
Carbohydrate Metabolism
Cholesterol / metabolism
Cholesterol Esters / metabolism
Elastin / metabolism*
Lipid Metabolism*
Phospholipids / metabolism
Triglycerides / metabolism
Reg. No./Substance:
0/Amino Acids; 0/Cholesterol Esters; 0/Phospholipids; 0/Triglycerides; 57-88-5/Cholesterol; 9007-58-3/Elastin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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