Document Detail

Elastin-like recombinamers as substrates for retinal pigment epithelial cell growth.
MedLine Citation:
PMID:  21442725     Owner:  NLM     Status:  MEDLINE    
The aim of this study is to investigate the use of elastin-like recombinamers (ELRs) as a substrate that can maintain the growth, phenotype, and functional characteristics of retinal pigment epithelial (RPE) cells efficiently and as a suitable carrier for the transplantation of autologous RPE cells for treatment of age-related macular degeneration (AMD). ELR films containing a bioactive sequence, RGD (ELR-RGD), and one with no specific sequence (ELR-IK) as control, were obtained by solvent-casting onto glass and subsequent cross-linking. ARPE19 cells were seeded on sterilized ELR films as well as on the control surfaces. Cells were analysed after 4, 24, 72, and 120 h to study cell adhesion, proliferation, cell viability, morphology, and specificity by staining with Trypan blue, DAPI, Rhodamin-Phalloidin and RPE65, ZO-1 antibodies and observing under fluorescence as well as electron microscope. ARPE19 cells seeded on both ELR films and controls were 100% viable and maintained their morphology and set of characteristics at the different time points studied. Cell proliferation on ELR-RGD was significantly higher than that found on ELR-IK at all time points, although it was less than the growth rate on polystyrene. ARPE19 cells grow well on ELR-RGD maintaining their phenotype. These results should be extended to further studies with fresh human RPE cells and in vivo studies to determine whether this ELR-RGD matrix could be used as a Bruch's membrane prosthesis and carrier for transplantation of RPE cells in patients suffering with AMD.
Girish K Srivastava; Laura Martín; Amar K Singh; Ivan Fernandez-Bueno; Manuel J Gayoso; Maria T Garcia-Gutierrez; Alessandra Girotti; Matilde Alonso; José C Rodríguez-Cabello; José C Pastor
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-03-25
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  97     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-04-19     Completed Date:  2011-08-15     Revised Date:  2012-05-28    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  243-50     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley Periodicals, Inc.
Institute of Applied Ophthalmobiology (IOBA), University of Valladolid, Valladolid, Spain.
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MeSH Terms
Amino Acid Sequence
Carrier Proteins / metabolism
Cell Adhesion / drug effects
Cell Line
Cell Proliferation / drug effects*
Elastin / genetics,  pharmacology*
Epithelial Cells / cytology
Eye Proteins / metabolism
Gene Expression
Macular Degeneration / therapy*
Molecular Sequence Data
Oligopeptides / genetics,  pharmacology*
Retinal Pigment Epithelium / cytology,  drug effects,  physiology*
Tissue Scaffolds / chemistry
Reg. No./Substance:
0/Carrier Proteins; 0/Eye Proteins; 0/Oligopeptides; 9007-58-3/Elastin; 99896-85-2/arginyl-glycyl-aspartic acid; EC isomerohydrolase

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