| Elastin-like recombinamers as substrates for retinal pigment epithelial cell growth. | |
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MedLine Citation:
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PMID: 21442725 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study is to investigate the use of elastin-like recombinamers (ELRs) as a substrate that can maintain the growth, phenotype, and functional characteristics of retinal pigment epithelial (RPE) cells efficiently and as a suitable carrier for the transplantation of autologous RPE cells for treatment of age-related macular degeneration (AMD). ELR films containing a bioactive sequence, RGD (ELR-RGD), and one with no specific sequence (ELR-IK) as control, were obtained by solvent-casting onto glass and subsequent cross-linking. ARPE19 cells were seeded on sterilized ELR films as well as on the control surfaces. Cells were analysed after 4, 24, 72, and 120 h to study cell adhesion, proliferation, cell viability, morphology, and specificity by staining with Trypan blue, DAPI, Rhodamin-Phalloidin and RPE65, ZO-1 antibodies and observing under fluorescence as well as electron microscope. ARPE19 cells seeded on both ELR films and controls were 100% viable and maintained their morphology and set of characteristics at the different time points studied. Cell proliferation on ELR-RGD was significantly higher than that found on ELR-IK at all time points, although it was less than the growth rate on polystyrene. ARPE19 cells grow well on ELR-RGD maintaining their phenotype. These results should be extended to further studies with fresh human RPE cells and in vivo studies to determine whether this ELR-RGD matrix could be used as a Bruch's membrane prosthesis and carrier for transplantation of RPE cells in patients suffering with AMD. |
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Authors:
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Girish K Srivastava; Laura Martín; Amar K Singh; Ivan Fernandez-Bueno; Manuel J Gayoso; Maria T Garcia-Gutierrez; Alessandra Girotti; Matilde Alonso; José C Rodríguez-Cabello; José C Pastor |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-03-25 |
Journal Detail:
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Title: Journal of biomedical materials research. Part A Volume: 97 ISSN: 1552-4965 ISO Abbreviation: J Biomed Mater Res A Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-04-19 Completed Date: 2011-08-15 Revised Date: 2012-05-28 |
Medline Journal Info:
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Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: United States |
Other Details:
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Languages: eng Pagination: 243-50 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Wiley Periodicals, Inc. |
Affiliation:
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Institute of Applied Ophthalmobiology (IOBA), University of Valladolid, Valladolid, Spain. girish@ioba.med.uva.es |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Carrier Proteins / metabolism Cell Adhesion / drug effects Cell Line Cell Proliferation / drug effects* Elastin / genetics, pharmacology* Epithelial Cells / cytology Eye Proteins / metabolism Gene Expression Humans Macular Degeneration / therapy* Molecular Sequence Data Oligopeptides / genetics, pharmacology* Regeneration* Retinal Pigment Epithelium / cytology, drug effects, physiology* Tissue Scaffolds / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Eye Proteins; 0/Oligopeptides; 9007-58-3/Elastin; 99896-85-2/arginyl-glycyl-aspartic acid; EC 3.1.1.64/retinoid isomerohydrolase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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