Document Detail


Elastic fiber assembly in the adult mouse pubic symphysis during pregnancy and postpartum.
MedLine Citation:
PMID:  22238283     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Impairment of pelvic organ support has been described in mice with genetic modifications of the proteins involved in elastogenesis, such as lysyl oxidase-like 1 (LOXL1) and fibulin 5. During pregnancy, elastic fiber-enriched pelvic tissues are modified to allow safe delivery. In addition, the mouse pubic symphysis is remodeled in a hormone-controlled process that entails the modification of the fibrocartilage into an interpubic ligament (IpL) and the relaxation of this ligament. After first parturition, recovery occurs to ensure pelvic tissue homeostasis. Because ligaments are the main supports of the pelvic organs, this study aimed to evaluate elastogenesis in the IpL during mouse pregnancy and postpartum. Accordingly, virgin, pregnant, and postpartum C57BL/6 mice were studied using light, confocal, and transmission electron microscopy as well as Western blots and real-time PCR. Female mice exhibited the separation of the pubic bones and the formation, relaxation, and postpartum recovery of the IpL. By the time the IpL was formed, the elastic fibers had increased in profile length and diameter, and they consisted of small conglomerates of amorphous material distributed among the bundles of microfibrils. Our analyses also indicated that elastin/tropoelastin, fibrillin 1, LOXL1/Loxl1, and fibulin 5 were spatially and temporally regulated, suggesting that these molecules may contribute to the synthesis of new elastic fibers during IpL development. Overall, this work revealed that adult elastogenesis may be important to assure the elasticity of the pelvic girdle during preparation for parturition and postpartum recovery. This finding may contribute to our understanding of pathological processes involving elastogenesis in the reproductive tract.
Authors:
Sílvio Roberto Consonni; Cláudio Chrysostomo Werneck; Débora Rodrigues Sobreira; Fabiana Kühne; Suzana Guimarães Moraes; Lúcia Elvira Alvares; Paulo Pinto Joazeiro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-17
Journal Detail:
Title:  Biology of reproduction     Volume:  86     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-18     Completed Date:  2012-09-17     Revised Date:  2012-12-06    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  151, 1-10     Citation Subset:  IM    
Affiliation:
Department of Histology and Embryology, State University of Campinas (Unicamp), Campinas, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Oxidoreductases / metabolism
Animals
Elastic Tissue / cytology,  metabolism*
Elasticity
Elastin / metabolism
Extracellular Matrix Proteins / metabolism
Female
Ligaments / cytology,  metabolism
Mice
Mice, Inbred C57BL
Microfilament Proteins / metabolism
Pelvis
Postpartum Period / metabolism*
Pregnancy
Pubic Symphysis / cytology,  metabolism*
Recombinant Proteins / metabolism
Chemical
Reg. No./Substance:
0/Extracellular Matrix Proteins; 0/Fbln5 protein, mouse; 0/Microfilament Proteins; 0/Recombinant Proteins; 0/fibrillin; 9007-58-3/Elastin; EC 1.4.-/Amino Acid Oxidoreductases; EC 1.4.3.-/Loxl1 protein, mouse

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