Document Detail

Elafin: an elastase-specific inhibitor of human skin. Purification, characterization, and complete amino acid sequence.
MedLine Citation:
PMID:  2394696     Owner:  NLM     Status:  MEDLINE    
A potent inhibitor of human leukocyte elastase (EC and porcine pancreatic elastase (EC was purified to homogeneity from human horny layers. It inhibits human leukocyte elastase and porcine pancreatic elastase in a 1:1 molar ratio and shows equilibrium dissociation constants of 6 x 10(-10) M and 1 x 10(-9) M, respectively. Inhibition of plasmin, trypsin, alpha-chymotrypsin, and cathepsin G was not observed. This inhibitor proved to be an acid stable basic peptide with an isoelectric point of 9.7. The complete amino acid sequence appears to be unique with 38% homology to the C-terminal half of antileukoprotease. The sequence shows that the inhibitor is composed of 57 amino acids and predicts a Mr of 7017. The high affinity as well as the apparent specificity for elastases suggests a functional role in preventing elastase-mediated tissue proteolysis. It is suggested that the term "elafin" be used to designate this inhibitor.
O Wiedow; J M Schröder; H Gregory; J A Young; E Christophers
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  265     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1990-10-09     Completed Date:  1990-10-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  14791-5     Citation Subset:  IM    
Department of Dermatology, University of Kiel, Federal Republic of Germany.
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MeSH Terms
Amino Acid Sequence
Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
Leukocytes / enzymology
Molecular Sequence Data
Molecular Weight
Pancreatic Elastase / antagonists & inhibitors*,  blood
Proteinase Inhibitory Proteins, Secretory
Sequence Homology, Nucleic Acid
Serine Proteinase Inhibitors / genetics,  isolation & purification*,  pharmacology
Skin / metabolism*
Reg. No./Substance:
0/Proteinase Inhibitory Proteins, Secretory; 0/Proteins; 0/Serine Proteinase Inhibitors; EC Elastase
Erratum In:
J Biol Chem 1991 Feb 15;266(5):3356

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