Document Detail


Either high-mannose-type or hybrid-type oligosaccharide is linked to the same asparagine residue in ovalbumin.
MedLine Citation:
PMID:  7284436     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
After pepsin digestion, all of the carbohydrates in ovalbumin were recovered in two glycopeptides, Glu-Glu-Lys-Tyr-Asn(CHO)-Leu-Thr-Ser-Val and Glu-Gln-Lys-Tyr-Asn(CHO)-Leu-Thr-Ser-Val. Almond glycopeptidase released quantitatively oligosaccharides from the glycopeptides. The products from both glycopeptides contained both the high-mannose-type oligosaccharides and the hybrid-type oligosaccharides in the same ratio. Thus, either the high-mannose-type or the hybrid-type oligosaccharide is attached to the unique asparagine residue in the ovalbumin molecule.
Authors:
H Ishihara; N Takahashi; J Ito; E Takeuchi; S Tejima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  669     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1981 Jul 
Date Detail:
Created Date:  1981-12-15     Completed Date:  1981-12-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  216-21     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amidohydrolases
Animals
Asparagine*
Chickens
Glycopeptides
Mannose / analysis
Oligosaccharides / analysis*
Ovalbumin*
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Plants / enzymology
Chemical
Reg. No./Substance:
0/Glycopeptides; 0/Oligosaccharides; 31103-86-3/Mannose; 7006-34-0/Asparagine; 9006-59-1/Ovalbumin; EC 3.5.-/Amidohydrolases; EC 3.5.1.52/Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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