| Eicosapentaenoic acid up-regulates apelin secretion and gene expression in 3T3-L1 adipocytes. | |
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MedLine Citation:
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PMID: 20352620 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent studies have shown the ability of apelin to restore glucose tolerance in obese and insulin-resistant mice. Eicosapentaenoic acid (EPA) is a polyunsaturated fatty acid (PUFA) from the omega-3 family that has many beneficial effects in obesity-linked disorders. The aim of this study was to examine in vitro the effects of EPA on apelin secretion and gene expression in mature 3T3-L1 adipocytes. Treatment with EPA (100 and 200 microM) significantly increased basal (p<0.01) and insulin-stimulated (p<0.001) apelin secretion and gene expression in adipocytes. EPA also stimulated Akt phosphorylation, a down-stream target of phosphatidylinositol 3-kinase (PI3K), in 3T3-L1 adipocytes. Moreover, treatment with the PI3K inhibitor LY294002 completely blocked EPA-stimulatory action on apelin mRNA gene expression (p<0.001), but not modified the stimulatory effect of EPA on basal apelin secretion. Furthermore, the stimulatory effect of EPA on basal apelin release was also observed in the presence of Actinomycin D and Cycloheximide, suggesting that EPA might also regulate apelin secretion by via post-transcriptional mechanisms. These findings suggest that the mechanisms mediating EPA-induced apelin synthesis and/or secretion are complex, involving steps that are PI3K dependent and steps that are PI3K independent. |
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Authors:
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Silvia Lorente-Cebrián; Matilde Bustos; Amelia Marti; José Alfredo Martinez; María Jesús Moreno-Aliaga |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular nutrition & food research Volume: 54 Suppl 1 ISSN: 1613-4133 ISO Abbreviation: Mol Nutr Food Res Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-10 Completed Date: 2010-09-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101231818 Medline TA: Mol Nutr Food Res Country: Germany |
Other Details:
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Languages: eng Pagination: S104-11 Citation Subset: IM |
Affiliation:
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Department of Nutrition, Food Science, Physiology and Toxicology, University of Navarra, Pamplona, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Adipocytes / drug effects, physiology* Animals Blotting, Western Carrier Proteins / genetics*, secretion Cycloheximide / pharmacology Eicosapentaenoic Acid / pharmacology* Gene Expression Regulation Mice Polymerase Chain Reaction / methods RNA / genetics, isolation & purification RNA, Messenger / drug effects, genetics |
| Chemical | |
Reg. No./Substance:
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0/Apln protein, mouse; 0/Carrier Proteins; 0/RNA, Messenger; 1553-41-9/Eicosapentaenoic Acid; 63231-63-0/RNA; 66-81-9/Cycloheximide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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