Document Detail


Eicosapentaenoic acid up-regulates apelin secretion and gene expression in 3T3-L1 adipocytes.
MedLine Citation:
PMID:  20352620     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent studies have shown the ability of apelin to restore glucose tolerance in obese and insulin-resistant mice. Eicosapentaenoic acid (EPA) is a polyunsaturated fatty acid (PUFA) from the omega-3 family that has many beneficial effects in obesity-linked disorders. The aim of this study was to examine in vitro the effects of EPA on apelin secretion and gene expression in mature 3T3-L1 adipocytes. Treatment with EPA (100 and 200 microM) significantly increased basal (p<0.01) and insulin-stimulated (p<0.001) apelin secretion and gene expression in adipocytes. EPA also stimulated Akt phosphorylation, a down-stream target of phosphatidylinositol 3-kinase (PI3K), in 3T3-L1 adipocytes. Moreover, treatment with the PI3K inhibitor LY294002 completely blocked EPA-stimulatory action on apelin mRNA gene expression (p<0.001), but not modified the stimulatory effect of EPA on basal apelin secretion. Furthermore, the stimulatory effect of EPA on basal apelin release was also observed in the presence of Actinomycin D and Cycloheximide, suggesting that EPA might also regulate apelin secretion by via post-transcriptional mechanisms. These findings suggest that the mechanisms mediating EPA-induced apelin synthesis and/or secretion are complex, involving steps that are PI3K dependent and steps that are PI3K independent.
Authors:
Silvia Lorente-Cebrián; Matilde Bustos; Amelia Marti; José Alfredo Martinez; María Jesús Moreno-Aliaga
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular nutrition & food research     Volume:  54 Suppl 1     ISSN:  1613-4133     ISO Abbreviation:  Mol Nutr Food Res     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-10     Completed Date:  2010-09-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101231818     Medline TA:  Mol Nutr Food Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  S104-11     Citation Subset:  IM    
Affiliation:
Department of Nutrition, Food Science, Physiology and Toxicology, University of Navarra, Pamplona, Spain.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Adipocytes / drug effects,  physiology*
Animals
Blotting, Western
Carrier Proteins / genetics*,  secretion
Cycloheximide / pharmacology
Eicosapentaenoic Acid / pharmacology*
Gene Expression Regulation
Mice
Polymerase Chain Reaction / methods
RNA / genetics,  isolation & purification
RNA, Messenger / drug effects,  genetics
Chemical
Reg. No./Substance:
0/Apln protein, mouse; 0/Carrier Proteins; 0/RNA, Messenger; 1553-41-9/Eicosapentaenoic Acid; 63231-63-0/RNA; 66-81-9/Cycloheximide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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