Document Detail


Eicosapentaenoic acid inhibits voltage-gated sodium channels and invasiveness in prostate cancer cells.
MedLine Citation:
PMID:  19154441     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND PURPOSE: The voltage-gated Na(+) channels (Na(v)) and their corresponding current (I(Na)) are involved in several cellular processes, crucial to metastasis of cancer cells. We investigated the effects of eicosapentaenoic (EPA), an omega-3 polyunsaturated fatty acid, on I(Na) and metastatic functions (cell proliferation, endocytosis and invasion) in human and rat prostate cancer cell lines (PC-3 and Mat-LyLu cells).
EXPERIMENTAL APPROACH: The whole-cell voltage clamp technique and conventional/quantitative real-time reverse transcriptase polymerase chain reaction analysis were used. The presence of Na(v) proteins was shown by immunohistochemical methods. Alterations in the fatty acid composition of phospholipids after treatment with EPA and metastatic functions were also examined.
KEY RESULTS: A transient inward Na(+) current (I(Na)), highly sensitive to tetrodotoxin, and Na(V) proteins were found in these cells. Expression of Na(V)1.6 and Na(V)1.7 transcripts (SCN8A and SCN9A) was predominant in PC-3 cells, while Na(V)1.7 transcript (SCN9A) was the major component in Mat-LyLu cells. Tetrodotoxin or synthetic small interfering RNA targeted for SCN8A and SCN9A inhibited metastatic functions (endocytosis and invasion), but failed to inhibit proliferation in PC-3 cells. Exposure to EPA produced a rapid and concentration-dependent suppression of I(Na). In cells chronically treated (up to 72h) with EPA, the EPA content of cell lipids increased time-dependently, while arachidonic acid content decreased. Treatment of PC-3 cells with EPA decreased levels of mRNA for SCN9A and SCN8A, cell proliferation, invasion and endocytosis.
CONCLUSION AND IMPLICATIONS: Treatment with EPA inhibited I(Na) directly and also indirectly, by down-regulation of Na(v) mRNA expression in prostate cancer cells, thus inhibiting their metastatic potential.
Authors:
T Nakajima; N Kubota; T Tsutsumi; A Oguri; H Imuta; T Jo; H Oonuma; M Soma; K Meguro; H Takano; T Nagase; T Nagata
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-16
Journal Detail:
Title:  British journal of pharmacology     Volume:  156     ISSN:  1476-5381     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-19     Completed Date:  2009-05-08     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  420-31     Citation Subset:  IM    
Affiliation:
Department of Ischemic Circulatory Physiology, The University of Tokyo, Japan. masamasa@pb4.so-net.ne.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line, Tumor
Cell Movement / drug effects
Cell Proliferation / drug effects
Eicosapentaenoic Acid / pharmacology*
Endocytosis / drug effects
Gene Expression / drug effects
Humans
Immunoblotting
Ion Channel Gating / drug effects*
Male
Neoplasm Invasiveness
Patch-Clamp Techniques
Prostatic Neoplasms / metabolism,  pathology*
RNA, Small Interfering / genetics
Rats
Reverse Transcriptase Polymerase Chain Reaction
Sodium Channels / biosynthesis,  genetics,  metabolism*
Transfection
Chemical
Reg. No./Substance:
0/RNA, Small Interfering; 0/Sodium Channels; 1553-41-9/Eicosapentaenoic Acid
Comments/Corrections

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