| Eicosapentaenoic acid and rosiglitazone increase adiponectin in an additive and PPARγ-dependent manner in human adipocytes. | |
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MedLine Citation:
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PMID: 20814411 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adiponectin, an anti-inflammatory and insulin-sensitizing protein secreted from adipose tissue, may be modulated by dietary fatty acids, although the mechanism is not fully known. Our objective was to investigate the effect of long-chain n-3 polyunsaturated fatty acids (PUFAs) on adiponectin in cultured human adipocytes, and to elucidate the role of peroxisome proliferator-activated receptor-γ (PPARγ) in this regulation. Isolated human adipocytes were cultured for 48 h with 100 µmol/l eicosapentaenoic acid (C20:5n-3, EPA), docosahexaenoic acid (C22:6n-3, DHA), palmitic acid (C16:0), 100 µmol/l EPA plus 100 µmol/l DHA, or bovine serum albumin (control). Additionally, adipocytes were treated for 48 h with a PPARγ antagonist (BADGE) or agonist (rosiglitazone) in isolation or in conjunction with either EPA or DHA. At 48 h, EPA and DHA increased (P < 0.05) adiponectin secretion by 88 and 47%, respectively, while EPA, but not DHA, also increased (136%, P < 0.001) cellular adiponectin protein. Interestingly, PPARγ antagonism completely abolished the DHA-mediated increase in secreted adiponectin, but only partially attenuated the EPA-mediated response. Thus, EPA's effects on adiponectin do not appear to be entirely PPARγ mediated. Rosiglitazone increased (P < 0.001) the secreted and cellular adiponectin protein (90 and 582%, respectively). Finally, the effects of EPA and rosiglitazone on adiponectin secretion were additive (+230% at 48 h combined, compared to 121 and 124% by EPA or rosiglitazone alone, respectively). Overall, our findings emphasize the therapeutic importance of long-chain n-3 PUFA alone, or in combination with a PPARγ agonist, as a stimulator of adiponectin, a key adipokine involved in obesity and related diseases. |
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Authors:
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Justine M Tishinsky; David W L Ma; Lindsay E Robinson |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-02 |
Journal Detail:
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Title: Obesity (Silver Spring, Md.) Volume: 19 ISSN: 1930-739X ISO Abbreviation: Obesity (Silver Spring) Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-31 Completed Date: 2011-04-29 Revised Date: 2012-08-13 |
Medline Journal Info:
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Nlm Unique ID: 101264860 Medline TA: Obesity (Silver Spring) Country: United States |
Other Details:
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Languages: eng Pagination: 262-8 Citation Subset: IM |
Affiliation:
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Department of Human Health and Nutritional Sciences, Animal Science and Nutrition Building, University of Guelph, Guelph, Ontario, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipocytes
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metabolism Adiponectin / secretion* Cells, Cultured Docosahexaenoic Acids / pharmacology Eicosapentaenoic Acid / pharmacology* Gene Expression Regulation / drug effects Humans Hypoglycemic Agents / pharmacology* PPAR gamma / agonists, antagonists & inhibitors, physiology* Thiazolidinediones / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Adiponectin; 0/Hypoglycemic Agents; 0/PPAR gamma; 0/Thiazolidinediones; 122320-73-4/rosiglitazone; 1553-41-9/Eicosapentaenoic Acid; 25167-62-8/Docosahexaenoic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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