Document Detail


Eicosapentaenoic acid prevents and reverses insulin resistance in high-fat diet-induced obese mice via modulation of adipose tissue inflammation.
MedLine Citation:
PMID:  20861209     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the effects of eicosapentaenoic acid (EPA) on prevention (P) and reversal (R) of high saturated-fat (HF) diet-induced obesity and glucose-insulin homeostasis. Male C57BL/6J mice were fed low-fat (LF; 10% energy from fat), HF (45% energy from fat), or a HF-EPA-P (45% energy from fat; 36 g/kg EPA) diet for 11 wk. A 4th group was initially fed HF for 6 wk followed by the HF-EPA-R diet for 5 wk. As expected, mice fed the HF diet developed obesity and glucose intolerance. In contrast, mice fed the HF-EPA-P diet maintained normal glucose tolerance despite weight gain compared with the LF group. Whereas the HF group developed hyperglycemia and hyperinsulinemia, both HF-EPA groups (P and R) exhibited normal glycemia and insulinemia. Further, plasma adiponectin concentration was lower in the HF group but was comparable in the LF and HF-EPA groups, suggesting a role of EPA in preventing and improving insulin resistance induced by HF feeding. Further analysis of adipose tissue adipokine levels and proteomic studies in cultured adipocytes indicated that dietary EPA supplementation of HF diets was associated with reduced adipose inflammation and lipogenesis and elevated markers of fatty acid oxidation. In C57BL/6J mice, EPA minimized saturated fat-induced insulin resistance and this is in part mediated by its effects on fatty acid oxidation and inflammation.
Authors:
Nishan S Kalupahana; Kate Claycombe; Shelley J Newman; Taryn Stewart; Nalin Siriwardhana; Nirupa Matthan; Alice H Lichtenstein; Naima Moustaid-Moussa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-09-22
Journal Detail:
Title:  The Journal of nutrition     Volume:  140     ISSN:  1541-6100     ISO Abbreviation:  J. Nutr.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-21     Completed Date:  2010-11-09     Revised Date:  2011-03-10    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1915-22     Citation Subset:  IM    
Affiliation:
Department of Animal Science, Obesity Research Center, University of Tennessee, Knoxville, TN 37996, USA.
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MeSH Terms
Descriptor/Qualifier:
3T3-L1 Cells
Adipokines / metabolism
Adiponectin / blood
Adipose Tissue / metabolism*
Animals
Biological Markers / blood,  metabolism
Body Weight
Dietary Fats / adverse effects*
Eicosapentaenoic Acid / administration & dosage*
Fatty Liver / pathology,  prevention & control
Gene Expression Regulation
Glucose Intolerance / blood,  prevention & control*
Hyperglycemia / blood,  prevention & control
Hyperinsulinism / blood,  prevention & control
Inflammation Mediators / blood,  metabolism*
Insulin Resistance*
Lipid Metabolism
Lipogenesis
Male
Mice
Mice, Inbred C57BL
Obesity / blood,  metabolism,  pathology,  prevention & control*
Chemical
Reg. No./Substance:
0/Adipokines; 0/Adiponectin; 0/Biological Markers; 0/Dietary Fats; 0/Inflammation Mediators; 1553-41-9/Eicosapentaenoic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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