Document Detail

Eicosapentaenoic Acid Attenuates Statin-Induced ER stress and Toxicity in Myoblast.
MedLine Citation:
PMID:  22749998     Owner:  NLM     Status:  Publisher    
We previously reported that eicosapentaenoic acid (EPA) improved statin-induced rhabdomyolysis in rats [Naba et al., 2006]. In this study, we report for the first time direct improvement by EPA of statin-induced toxicity in cultured myoblasts and the mechanistic involvement of endoplasmic reticulum (ER) stress. Differentiated rhabdomyosarcoma cells (RD cells) were treated with statins and EPA for 1-4 days. Statins induced various toxic changes in RD cells, and EPA attenuated all of these changes. Interestingly, statins increased mRNA expression of ER stress markers (XBP-1 and CHOP) and EPA attenuated both. Further, in a statin-induced rat model of rhabdomyolysis, these markers in skeletal muscle were significantly correlated with plasma CPK activity. In RD cells, statins also increased p-c-Jun protein content and caspase-3/7 activity, while 4-PBA, an ER stress attenuator, PPAR-δ agonist, and EPA attenuated them. These findings suggest that EPA attenuates statin-induced ER stress, JNK activation and toxicity in cultured myoblast cells, and that PPAR-δ may mechanically involved in the effects of EPA.
Masahiko Ohta; Hiroyuki Kawano; Tatsuto Notsu; Hiroyasu Naba; Kazunori Imada
Related Documents :
7564508 - Intracellular pharmacokinetics of 2-chlorodeoxyadenosine in leukemia cells from patient...
11336348 - Characterization of the amino acid transport of new immortalized choroid plexus epithel...
20226858 - Improved membrane transport of astaxanthine by liposomal encapsulation.
16666418 - L-glutamate-dependent medium alkalinization by asparagus mesophyll cells : cotransport ...
22966858 - The small gtpase rhog mediates glioblastoma cell invasion.
12072178 - Regulation and measurement of oxidative stress in apoptosis.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-27
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  -     ISSN:  1090-2104     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-7-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Inc.
Pharmaceutical Research Center, Mochida Pharmaceutical Co., Ltd., 722 Uenohara, Jimba, Gotemba 412-8524, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Phosphorylation of elongation factor-2 kinase differentially regulates the enzyme's stability under ...
Next Document:  C-terminal truncation of Vascular Endothelial Growth Factor mimetic helical peptide preserves struct...