Document Detail


Eicosanoids mediate melanotic nodulation reactions to viral infection in larvae of the parasitic wasp, Pimpla turionellae.
MedLine Citation:
PMID:  17904576     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nodulation is the quantitatively predominant insect cellular immune function activated in response to bacterial, fungal and some viral infections. We posed the hypothesis that parasitoid insects express melanotic nodulation reactions to viral challenge and that eicosanoids mediate these reactions. Treating fifth-instar larvae of the ichneumonid endoparasitoid Pimpla turionellae with Bovine Herpes Simplex Virus-1 (BHSV-1) induced nodulation reactions in a challenge dose-dependent manner. Experimental larvae treated with the cyclooxygenase inhibitor, indomethacin, the lipoxygenase inhibitor, esculetin, and the phospholipase A2 inhibitor, dexamethasone, resulted in severely impaired nodulation reactions to our standard BHSV-1 challenge dose. The immunoinhibitory influence of dexamethasone was reversed in larvae reared on culture medium amended with arachidonic acid, the fatty acid precursor of eicosanoid biosynthesis. Larvae that had been reared on media amended with indomethacin, esculetin, or dexamethasone were also compromised in their nodulation reactions to viral challenge. The influence of the orally administered pharmaceutical was expressed in a dose-dependent manner. Finally, wasp larvae reared in the presence of indomethacin and dexamethasone expressed significantly decreased levels of phenoloxidase activity in response to viral challenge. These findings draw attention to the idea that endoparasitoid insects express cellular immune reactions to viral challenge; they also support our hypothesis that eicosanoids mediate nodulation reactions to viral challenge in these highly specialized insects.
Authors:
Yonca Durmuş; Ender Büyükgüzel; Burcin Terzi; Hasan Tunaz; David Stanley; Kemal Büyükgüzel
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2007-07-29
Journal Detail:
Title:  Journal of insect physiology     Volume:  54     ISSN:  0022-1910     ISO Abbreviation:  J. Insect Physiol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-21     Completed Date:  2008-04-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985080R     Medline TA:  J Insect Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  17-24     Citation Subset:  IM    
Affiliation:
Department of Biology, Faculty of Arts and Science, Karaelmas University, Zonguldak, Turkey.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Arachidonic Acids / pharmacology
Cyclooxygenase Inhibitors / toxicity
Dexamethasone / toxicity
Dose-Response Relationship, Drug
Eicosanoids / immunology*,  metabolism
Herpesvirus 1, Bovine / immunology
Immunity, Cellular / drug effects,  immunology*
Indomethacin / toxicity
Larva / immunology*,  virology
Monophenol Monooxygenase / metabolism
Spectrophotometry, Ultraviolet
Umbelliferones / toxicity
Wasps / immunology*,  virology
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Cyclooxygenase Inhibitors; 0/Eicosanoids; 0/Umbelliferones; 305-01-1/esculetin; 50-02-2/Dexamethasone; 53-86-1/Indomethacin; EC 1.14.18.1/Monophenol Monooxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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