Document Detail


Efflux of organic acids in Penicillium simplicissimum is an energy-spilling process, adjusting the catabolic carbon flow to the nutrient supply and the activity of catabolic pathways.
MedLine Citation:
PMID:  11932458     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Continuous cultivation was used to study the effect of glucose, ammonium, nitrate or phosphate limitation on the excretion of tricarboxylic acid (TCA) cycle intermediates by Penicillium simplicissimum. Additionally, the effect of benzoic acid, salicylhydroxamic acid (SHAM) and 2,4-dinitrophenol on TCA cycle intermediates was studied. The physiological state of the fungus was characterized by its glucose and O(2) consumption, its CO(2) production, its intra- and extracellular concentrations of TCA cycle intermediates, as well as by its biomass yield, its maintenance coefficient and its respiratory quotient. The excretion of TCA cycle intermediates was observed during ammonium-, nitrate- and phosphate-limited growth. The highest productivity was found with phosphate-limited growth. The respiratory quotient was 1.3 under ammonium limitation and 0.7 under phosphate limitation. Citrate was always the main excreted intermediate. This justifies calling this excretion an energy-spilling process, because citrate excretion avoids the synthesis of too much NADH. The addition of benzoic acid further increased the excretion of TCA cycle intermediates by ammonium-limited hyphae. A SHAM-sensitive respiration was constitutively present during ammonium-limited growth of the fungus. The sum of the excreted organic acids was negatively correlated with the biomass yield (Y(GlcX)).
Authors:
Martin Gallmetzer; Wolfgang Burgstaller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  148     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-04-04     Completed Date:  2002-07-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  1143-9     Citation Subset:  IM    
Affiliation:
Institute of Microbiology, Leopold-Franzens-University of Innsbruck, Technikerstrasse 25, 6020 Innsbruck, Austria. martin.gallmetzer@uibk.ac.at
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MeSH Terms
Descriptor/Qualifier:
Benzoic Acid / pharmacology
Citric Acid Cycle
Fungi / metabolism
Glucose / metabolism
Kinetics
Nitrates / metabolism
Oxygen Consumption
Penicillium / drug effects,  growth & development,  metabolism*
Phosphates / metabolism
Quaternary Ammonium Compounds / metabolism
Species Specificity
Tricarboxylic Acids / metabolism*
Chemical
Reg. No./Substance:
0/Nitrates; 0/Phosphates; 0/Quaternary Ammonium Compounds; 0/Tricarboxylic Acids; 50-99-7/Glucose; 65-85-0/Benzoic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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