Document Detail


Efflux of bile acids in Lactobacillus reuteri is mediated by ATP.
MedLine Citation:
PMID:  21744273     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To study whether an active bile acid (BA) efflux occurs in Lactobacillus reuteri CRL 1098 as well as the nature (ATP or proton motive force [PMF] mediated primary transport) of the BA extrusion mechanism. BAs are powerful detergents which disorganize the lipid bilayer structure of cellular membranes. Specific bile resistance mechanisms (bile efflux, bile salt hydrolysis, and intrinsic architecture and composition of cell membrane the most prevalent) have been described in intestinal bacteria. L. reuteri, showed a significant degree of resistance to the toxic action of BA and the presence of an active efflux ATP-dependent of conjugated (taurocholic [TCA]) and free (cholic [CA]) BA in the CRL 1098 strain is now reported. However, due the high pKa (5.5) of cholic acid (CA) compared with the conjugated species, a significant fraction (between 35 and 50% at pH 6.5 and 5.2, respectively) of free BA also diffused passively, even in the absence of ATP. To our knowledge, our results represent the first evidence of ATP as the energy source involved in the BA extrusion in L. reuteri.
Authors:
Ana Yanina Bustos; Raúl Raya; Graciela Font de Valdez; María Pía Taranto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-07-10
Journal Detail:
Title:  Biotechnology letters     Volume:  33     ISSN:  1573-6776     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-09-28     Completed Date:  2012-01-19     Revised Date:  2013-07-15    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  2265-9     Citation Subset:  IM    
Affiliation:
Centro de Referencia para Lactobacilos (CERELA-CONICET)-Chacabuco 145 (T4000ILC), San Miguel de Tucumán, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism*
Biological Transport, Active
Cholic Acid / metabolism*,  toxicity
Lactobacillus reuteri / drug effects,  metabolism*
Taurocholic Acid / metabolism*,  toxicity
Chemical
Reg. No./Substance:
56-65-5/Adenosine Triphosphate; 81-24-3/Taurocholic Acid; 81-25-4/Cholic Acid

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