| Efflux mechanisms at the developing brain barriers: ABC-transporters in the fetal and postnatal rat. | |
| | |
MedLine Citation:
|
PMID: 20466047 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Proteins of the ATP-binding cassette (ABC) family, present at the blood-brain barrier interfaces, have been shown to reduce the entry of compounds from blood into the brain by active efflux. Their substrates are diverse including many drugs and toxins and therefore provide an important mechanism for brain neuroprotection. However, knowledge of their presence and function in the developing brain is very limited. We have used qPCR and immunocytochemistry to determine gene expression and localisation of four main barrier ABC-transporters (pgp/ABCB1, MRP1/ABCC1, MRP4/ABCC4 and BCRP/ABCG2) in the fetal and neonatal rat brain cerebral blood vessels (site of blood-brain barrier) and choroid plexus (site of blood-CSF barrier). The study shows that ABC-transporters localise to the brain barriers even at early fetal stages and although pgp expression was lower in the fetus, the other transporters were expressed at comparable levels in fetal and adult brains suggesting direct neuroprotection of the brain in addition to that provided by the placenta. BCRP was expressed at higher levels in developing choroid plexus and was only detected at fetal stages on the blood-facing side of epithelial cells indicating a particular role of this transporter for early brain efflux mechanisms. |
| | |
Authors:
|
C Joakim Ek; Annamae Wong; Shane A Liddelow; Pia A Johansson; Katarzyna M Dziegielewska; Norman R Saunders |
Related Documents
:
|
3364297 - The analysis of po2 difference between air space and arterialized blood in chicken eggs... 10087007 - Orexin a but not orexin b rapidly enters brain from blood by simple diffusion. 457877 - Osmotic blood-brain barrier disruption. computerized tomographic monitoring of chemothe... 6709937 - Ocular responses to leukotriene c4 and d4 in the cat. 8484247 - Taiwan blood donation program: a model for development. 12168277 - Icp and cbf regulation: effect of the decompressive craniectomy. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-11 |
Journal Detail:
|
Title: Toxicology letters Volume: 197 ISSN: 1879-3169 ISO Abbreviation: Toxicol. Lett. Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-07-08 Completed Date: 2010-07-30 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7709027 Medline TA: Toxicol Lett Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 51-9 Citation Subset: IM |
Copyright Information:
|
Copyright 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
|
Department of Pharmacology, University of Melbourne, Parkville, Australia. cjek@unimelb.edu.au |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
ATP-Binding Cassette Transporters
/
genetics,
metabolism* Animals Biological Transport, Active Blood-Brain Barrier / cytology, growth & development, metabolism* Fetus / metabolism* Multidrug Resistance-Associated Proteins / metabolism P-Glycoprotein / metabolism RNA, Messenger / metabolism Rats |
| Chemical | |
Reg. No./Substance:
|
0/ATP-Binding Cassette Transporters; 0/Multidrug Resistance-Associated Proteins; 0/P-Glycoprotein; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: 7-Ketocholesterol and cholesterol-5alpha,6alpha-epoxide induce smooth muscle cell migration and prol...
Next Document: Burden of cadmium in early childhood; longitudinal assessment of urinary cadmium in rural Bangladesh...