Document Detail


Efficient transformation of chicken embryo fibroblasts by c-Jun requires structural modification in coding and noncoding sequences.
MedLine Citation:
PMID:  2123464     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To assess the transforming capability of the c-Jun protein, we introduced the chicken c-jun proto-oncogene into a replication competent avian retroviral expression vector (RCAS). Viral Jun efficiently transformed chicken embryo fibroblasts (CEFs) when expressed from this vector. Overexpression of c-Jun leads to transformation of CEFs with an efficiency that is 15- to 25-fold less than that seen for v-Jun, suggesting that v-Jun contains structural features that increase its oncogenic potential relative to c-Jun. There are four structural differences between v-Jun and c-Jun. To determine the relative contribution that each of these structural differences between v-Jun and c-Jun has on oncogenic activity, several deletion and substitution mutants were constructed. Each of these mutants was expressed in CEF and assayed for transformation by focus formation. Analysis of the results reveals that deletion of a region of 27 amino acids near the amino terminus of c-Jun and deletion of 3'-untranslated sequences are critical in activating the full oncogenic potential of Jun.
Authors:
T J Bos; F S Monteclaro; F Mitsunobu; A R Ball; C H Chang; T Nishimura; P K Vogt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genes & development     Volume:  4     ISSN:  0890-9369     ISO Abbreviation:  Genes Dev.     Publication Date:  1990 Oct 
Date Detail:
Created Date:  1991-01-16     Completed Date:  1991-01-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1677-87     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Southern California, School of Medicine, Los Angeles 90033.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cell Transformation, Neoplastic / genetics*
Chick Embryo
Chromosome Deletion
DNA-Binding Proteins / genetics*,  physiology
Fibroblasts / physiology
Genetic Code / physiology
Genetic Vectors
Molecular Sequence Data
Mutation / genetics
Proto-Oncogene Proteins / genetics*,  physiology
Proto-Oncogene Proteins c-jun
Transcription Factors / genetics*,  physiology
Transfection / genetics
Grant Support
ID/Acronym/Agency:
CA 42564/CA/NCI NIH HHS; CA51982/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-jun; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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