Document Detail


Efficient transformation of Lactococcus lactis IL1403 and generation of knock-out mutants by homologous recombination.
MedLine Citation:
PMID:  17518422     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lactococcus lactis IL1403 is a Gram-positive bacterium of great biotechnological interest for food grade applications. Its use is however hampered by the difficulty to efficiently transform this strain. We here describe a detailed, optimized electrotransformation protocol which yields a transformation efficiency of 10(6) cfu/microg of DNA with the two E. coli Gram-positive shuttle vectors pC3 and pVA838. The utility of the protocol was demonstrated by the generation of single- and double-knock-out mutants by homologous recombination.
Authors:
Simon D Gerber; Marc Solioz
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of basic microbiology     Volume:  47     ISSN:  0233-111X     ISO Abbreviation:  J. Basic Microbiol.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-28     Completed Date:  2007-10-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8503885     Medline TA:  J Basic Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  281-6     Citation Subset:  IM    
Affiliation:
Department of Clinical Pharmacology, University of Berne, Berne, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Electroporation / methods*
Genetic Vectors
Lactococcus lactis / genetics*
Mutation
Transformation, Bacterial*

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