| Efficient siRNA Delivery Using a Polyamidoamine Dendrimer with a Modified Pentaerythritol Core. | |
| | |
MedLine Citation:
|
PMID: 22274556 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
PURPOSE: Delivery of siRNA into cells remains a critical challenge. Our lab has shown a novel polyamidoamine (PAMAM) dendrimer with modified pentaerythritol derivative core (PD dendrimer) to exhibit high plasmid DNA transfection efficiency and low cytotoxicity. Here, we evaluate PD dendrimer as a siRNA carrier. METHODS: Agarose gel electrophoresis and AFM were used to confirm formation of generation 5 (G5)-PD dendrimer/siRNA nanoparticles (NPs). G5 PD dendrimer/anti-luciferase siRNA NPs were used to transfect SK Hep-1 cells with stable luciferase expression. Effects of various endocytic pathway inhibitors on uptake of G5 PD dendrimer/siRNA NPs in SK Hep-1 cells were also investigated. RESULTS: Agarose gel electrophoresis indicated that G5 PD dendrimer and siRNA formed NPs at weight ratios >0.5:1. G5 PD dendrimer showed effective luciferase gene silencing when weight ratio was 3.0:1 and above. Treatment with endocytosis inhibitors showed that clathrin-mediated endocytosis was the main endocytic pathway by which G5-PD dendrimer/siRNA NPs enter the cell. CONCLUSIONS: These results show that the novel G5 PD dendrimer has high siRNA delivery activity and is promising as a delivery agent for its therapeutic application. |
| | |
Authors:
|
Yue Zhang; Chenguang Zhou; Kwang Joo Kwak; Xinmei Wang; Bryant Yung; L James Lee; Yanming Wang; Peng George Wang; Robert J Lee |
Related Documents
:
|
19649226 - Hibernating myocardium: programmed cell survival or programmed cell death? 19620776 - Engaging the lysosomal compartment to combat b cell malignancies. 15708436 - Altered transcriptional regulators in response to serum in immortalized lymphocytes fro... 16799806 - The positional distribution of cell death of ray parenchyma in a conifer, abies sachali... 1280756 - Porcine endometrial epithelial cells immortalized by transfection with origin-defective... 6317646 - Inhibition of lateral wall elongation by mecillinam stimulates cell division in certain... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-25 |
Journal Detail:
|
Title: Pharmaceutical research Volume: - ISSN: 1573-904X ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-1-25 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8406521 Medline TA: Pharm Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
College of Pharmacy, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Formulation and in vitro characterization of composite biodegradable magnetic nanoparticles for magn...
Next Document: Targeted Endothelial Gene Delivery by Ultrasonic Destruction of Magnetic Microbubbles Carrying Lenti...