| Efficient purification of somatomedin-C/insulin-like growth factor I using immunoaffinity chromatography. | |
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MedLine Citation:
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PMID: 3882085 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Somatomedin-C/insulin-like growth factor I was purified from human plasma using a monoclonal antibody affinity column. Combining immunoaffinity chromatography with standard protein purification methods resulted in an overall recovery of 18%. The 35 micrograms of somatomedin-C/insulin-like growth factor I purified from 500 ml of plasma appeared as a single band when analyzed by polyacrylamide gel electrophoresis and could be used in radioimmunoassay and receptor binding studies. |
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Authors:
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S D Chernausek; P G Chatelain; M E Svoboda; L E Underwood; J J Van Wyk |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 126 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1985 Jan |
Date Detail:
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Created Date: 1985-03-20 Completed Date: 1985-03-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 282-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Chromatography, Affinity / methods Chromatography, Liquid Electrophoresis, Polyacrylamide Gel Humans Insulin / isolation & purification* Insulin-Like Growth Factor I Isoelectric Focusing Peptides / isolation & purification* Somatomedins / isolation & purification* |
| Grant Support | |
ID/Acronym/Agency:
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5RO1AM01022-28/AM/NIADDK NIH HHS; RR05408-21/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Peptides; 0/Somatomedins; 11061-68-0/Insulin; 67763-96-6/Insulin-Like Growth Factor I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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