Document Detail


Efficient purification of native recombinant proteins using proteases immobilized on cellulose.
MedLine Citation:
PMID:  22265893     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cellulose binding domain (CBD) fusion protease was employed to digest the CBD fusion protein. After cleavage, the tag free target proteins can be separated from the CBD tag and CBD fusion protease which still adsorbed to the cellulose by centrifugation. The green fluorescent protein was efficiently purified using this method.
Authors:
Dongming Lan; Yan Tai; Yanfei Shen; Fanghua Wang; Bo Yang; Yonghua Wang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-20
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  -     ISSN:  1347-4421     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
Affiliation:
School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, PR China.
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