Document Detail


Efficient purification of GP-1D8 glycoprotein from human breast carcinoma tissue by immunoaffinity chromatography.
MedLine Citation:
PMID:  18800296     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Anti-GP-1D8 monoclonal antibody was produced in our laboratory. Immunoaffinity purification of GP-1D8 glycoprotein from human breast carcinoma tissues, on column with the monospecific antibody, is developed. The procedure permits purification of GP-1D8 to a highly purified state. It appeared as a single band in sodium dodcyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular mass of GP-1D8 was determined to be 66 kDa by mass spectrometry. Its antigenicity was stable between 0 and 70 degrees C. It was breast cancer specific, as determined by immunohistochemistry and immunocytochemistry. The preparation of anti-GP-1D8 monoclonal antibody will facilitate further detection of, and functional study on, GP-1D8. The study also provides a simple, economical, and efficient method for affinity purification of target proteins from human or animal tissues.
Authors:
Renqing Feng; Zhenquan Guo; Jinfeng Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Preparative biochemistry & biotechnology     Volume:  38     ISSN:  1532-2297     ISO Abbreviation:  Prep. Biochem. Biotechnol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-09-18     Completed Date:  2008-11-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9607037     Medline TA:  Prep Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  321-33     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, College of Life Sciences, Peking University, Beijing, P R China. rqfeng@pku.edu.cn
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / immunology
Breast Neoplasms / metabolism*
Cell Fusion
Chromatography, Affinity / methods*
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Glycoproteins / immunology*,  isolation & purification*
Humans
Immunohistochemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/GP-1D8 glycoprotein, human; 0/Glycoproteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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