| Efficient propagation of betanodavirus in a murine astrocytoma cell line. | |
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MedLine Citation:
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PMID: 18556083 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Betanodavirus, a bipartite RNA virus of fishes and a member of Nodaviridae family, targets nervous tissues and is the causative agent of viral nervous necrosis in marine farmed fish. Betanodavirus is thought to be propagated only in fish cells because betanodavirus has only been isolated in fish and it is not well propagated in mammalian culture cells. However, the host specificity of betanodavirus has not yet been well analyzed. To analyze the host specificity of betanodavirus, various mammalian cells were screened for their permissiveness to betanodavirus. As a result, redspotted grouper nervous necrosis virus can be propagated efficiently in the murine astrocytoma cell line, DBT. The level of viral production in DBT was 10-fold-higher than in the fish cell line, E-11. This result is the first to demonstrate the efficient propagation of betanodavirus in mammalian cells and may help to elucidate the mechanism of the host specificity of betanodavirus. |
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Authors:
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Naoki Takizawa; Kei Adachi; Tohru Ichinose; Nobuyuki Kobayashi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-06-11 |
Journal Detail:
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Title: Virus research Volume: 136 ISSN: 0168-1702 ISO Abbreviation: Virus Res. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-07-08 Completed Date: 2008-09-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8410979 Medline TA: Virus Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 206-10 Citation Subset: IM |
Affiliation:
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Laboratory of Molecular Biology of Infectious Agents, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Astrocytoma / virology* Cell Culture Techniques Cell Line Mice Nodaviridae / growth & development* Virology / methods* |
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